Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,896,621	A→G	D147D (GAT→GAC)	bglB ←	6‑phospho‑beta‑glucosidase BglB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,896,621	0	A	G	100.0%	39.6 / NA	15	D147D (GAT→GAC)	bglB	6‑phospho‑beta‑glucosidase BglB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/5);  total (10/5)

GTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATA  >  NZ_CP009273/3896540‑3896691                                                                                  |                                                                       gTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGAc                                                                >  2:383876/1‑90 (MQ=255)    gATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGc                                                             <  1:327865/90‑1 (MQ=255)    gATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGc                                                             >  2:123956/1‑90 (MQ=255)      tttCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTc                                                           >  2:220311/1‑90 (MQ=255)      tttCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTc                                                           >  2:80026/1‑90 (MQ=255)       ttCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCg                                                          >  2:230332/1‑90 (MQ=255)                                   ttATGTGGGTAGGGAGTAAAGCCCGGGCGGGCGTAATGCTCGAAGTGGTCGATGCCCGCTCGATTAGCCCAACCGCCGTAGTTTTTCAcc                              <  1:7975/90‑1 (MQ=255)                                     ttgtgGGTGGGGAGAAAAGCCCGGGGGGGCGTAATGCTCGAGGGGGTCGATGCCCGCTCGATTACCCCACCCGCCGTAGTTTTTCACCAg                            <  2:85205/89‑1 (MQ=255)                                              gCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATgg                   >  1:191483/1‑90 (MQ=255)                                                        cccGTGGGGGCGTAATGCTCGAAGGGGTCGATGCCCGCTCGATTACCCAAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTaa         <  1:52181/89‑1 (MQ=255)                                                          cGTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGAAGTTTTTCACCAGCCCATATGGCATTTCGTAATg       >  1:229931/1‑90 (MQ=255)                                                           gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg      >  1:239094/1‑90 (MQ=255)                                                           gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg      >  2:254780/1‑90 (MQ=255)                                                           gTGCGGGCGTAATGCTCGAAGTGGTCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATgg      >  2:77019/1‑90 (MQ=255)                                                               gggCGTAATCCTCGAGGTGGTCGATCCCCGCTCAATTACCCCACCCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATa  <  2:180753/90‑1 (MQ=255)                                                                                  |                                                                       GTTGATTTCATTAAACGTCAGCCATAACGCCACTTTATGTTGGTAGCGAGTAAAGACCGTGCGGGCGTAATGCTCGAAGTGATCGATGACCGCTCGATTAGCCCAACCGCCGTAGTTTTTCACCAGCCCATATGGCATTTCGTAATGGGATA  >  NZ_CP009273/3896540‑3896691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑