Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,215,724	T→G	W657G (TGG→GGG)	metH →	methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,215,724	0	T	G	100.0%	32.6 / NA	11	W657G (TGG→GGG)	metH	methionine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (4/7);  total (4/7)

GAGCGTTTACTGGAGCTTGCCGAGAAATATCGCGGCAGCAAAACCGACGACACCGCCAACGCCCAGCAGGCGGAGTGGCGCTCGTGGGAAGTGAATAAACGTCTGGAATACTCGCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCA  >  NZ_CP009273/4215640‑4215809                                                                                     |                                                                                      gAGCGTTTACTGGAGCTTGCCGAGAAATATCGCGGCAGCAAAACCGACGACACCGCCAACGCCCAGCAGGCGGAGTGGCGCTCGGGGGaa                                                                                  >  2:400836/1‑90 (MQ=255)  aGCGTTTACTGGAGCTTGCCGAGAAATATCGCGGCAGCAAAACCGACGACACCGCCAACGCCCAGCAGGCGGAGTGGCGCTCGGGGGAAg                                                                                 <  1:80967/90‑1 (MQ=255)         aCTGGAGCTTGCCGAGAAATATCGCGGCAGCAAAACCGACGACACCGCCAACGCCCAGCAGGCGGAGTGGCGCTCGGGGGAAGTGAATaa                                                                          <  1:98462/90‑1 (MQ=255)                    ccGAGAAATATCGCGGCAGCAAAACCGACGACACCGCCAACGCCCAGCAGGCGGAGTGGCGCTCGGGGGAAGTGAATAAACGTCTGGAAt                                                               <  1:202489/90‑1 (MQ=255)                                                             gCCCAGCAGGCGGAGTGGCGCTCGGGGGAAGTGAATaa                                                                          <  1:177963/38‑1 (MQ=38)                                                             gCCCAGCAGGCGGAGTGGCGCTCGGGGGAAGTGAATaa                                                                          >  2:177963/1‑38 (MQ=255)                                                               ccAGCAGGCGGAGTGGCGCTCGGGGGAAGTGAATAAACGTCTGGAATACTCGCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATAc                    <  1:384486/90‑1 (MQ=255)                                                                 agcagGCGGAGTGGCGCTCGGGGGAAGTGAATAAACGTCTGGAATACTCGCTGGTCAAAGGCATTAcc                                        >  1:234767/1‑68 (MQ=255)                                                                 agcagGCGGAGTGGCGCTCGGGGGAAGTGAATAAACGTCTGGAATACTCGCTGGTCAAAGGCATTAcc                                        <  2:234767/68‑1 (MQ=255)                                                                           gTGGCGCTCGGGGGAAGTGAATAAACGTCTGGAATACTCGCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCg        >  2:234387/1‑90 (MQ=255)                                                                                 cTCGGGGGAAGTGAATAAACGTCTGGAATACTCGCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCcagca  <  2:351273/90‑1 (MQ=255)                                                                                     |                                                                                      GAGCGTTTACTGGAGCTTGCCGAGAAATATCGCGGCAGCAAAACCGACGACACCGCCAACGCCCAGCAGGCGGAGTGGCGCTCGTGGGAAGTGAATAAACGTCTGGAATACTCGCTGGTCAAAGGCATTACCGAGTTTATCGAGCAGGATACCGAAGAAGCCCGCCAGCA  >  NZ_CP009273/4215640‑4215809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑