Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,543,103	A→G	E129G (GAA→GGA)	uxuB →	fructuronate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,543,103	0	A	G	100.0%	36.6 / NA	12	E129G (GAA→GGA)	uxuB	fructuronate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/8);  total (4/8)

ATTCTCAACGCGATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCCGCTGATCAAGCACGATCTGGAAAACCCGACTG  >  NZ_CP009273/4543048‑4543186                                                        |                                                                                    aTTCTCAACGCGATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGGAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCaa                                                   >  2:245279/1‑90 (MQ=255)       aaCGCGATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGGAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGAt                                             <  1:109209/90‑1 (MQ=255)           cgATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGGAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCa                                         >  2:89721/1‑90 (MQ=255)                  gcgTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGGAAAAg                                                                                >  1:64555/1‑44 (MQ=255)                  gcgTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGGAAAAg                                                                                <  2:64555/44‑1 (MQ=255)                    gTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGGAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCCGc                                <  1:245279/90‑1 (MQ=255)                    gTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGGAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCCGc                                <  1:385617/90‑1 (MQ=255)                    gTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGGAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCCGc                                <  2:390498/90‑1 (MQ=255)                                        tctctAACGGTCACGGGAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCCGCTGATCAAGCACGATCTGGaa            <  1:73127/90‑1 (MQ=255)                                                  tCACGGGAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCCGCTGATCAAGCACGATCTGGAAAACCCGACTg  <  2:174505/90‑1 (MQ=255)                                                   cACGGGAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACt                                                 <  1:149648/42‑1 (MQ=255)                                                   cACGGGAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACt                                                 >  2:149648/1‑42 (MQ=255)                                                        |                                                                                    ATTCTCAACGCGATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCGCTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCCGCTGATCAAGCACGATCTGGAAAACCCGACTG  >  NZ_CP009273/4543048‑4543186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑