Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	551,422	C→G	G452G (GGC→GGG)	cysS →	cysteine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	551,422	0	C	G	100.0%	33.3 / NA	11	G452G (GGC→GGG)	cysS	cysteine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (8/3);  total (8/3)

TGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCC  >  NZ_CP009273/551337‑551501                                                                                      |                                                                                tGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGGCCGc                                                                             >  1:132745/1‑90 (MQ=255)   gATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGGCCGCaa                                                                           <  1:34276/90‑1 (MQ=255)                         gggCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGGCCGCAAGGGACCACCTGGcgt                                                            >  1:44405/1‑83 (MQ=255)                         gggCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGGCCGCAAGGGACCACCTGGcgt                                                            <  2:44405/83‑1 (MQ=255)                              gcggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGGCCGCAAGGGACCACCTGGCGTCGTAAGTAATTg                                                >  1:177519/1‑90 (MQ=255)                                                              gaTGGGGATCGTGCTGGAAGATGGGCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCgg                >  1:253858/1‑90 (MQ=255)                                                                        gTGCTGGAAGATGGGCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTg      >  1:268979/1‑90 (MQ=255)                                                                        gTGCTGGAAGATGGGCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTg      >  2:118200/1‑90 (MQ=255)                                                                           cTGGAAGATGGGCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTgcgc   >  1:83315/1‑90 (MQ=255)                                                                            tGGAAGATGGGCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGcc  >  1:165566/1‑90 (MQ=255)                                                                            tGGAAGATGGGCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGcc  <  2:86154/90‑1 (MQ=255)                                                                                      |                                                                                TGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCC  >  NZ_CP009273/551337‑551501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑