Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	860,269	A→G	V106A (GTC→GCC)	moeB ←	molybdopterin‑synthase adenylyltransferase MoeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	860,269	0	A	G	100.0%	29.4 / NA	10	V106A (GTC→GCC)	moeB	molybdopterin‑synthase adenylyltransferase MoeB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/7);  total (3/7)

ACGTTATCCGTACAGTCGAGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCAT  >  NZ_CP009273/860191‑860350                                                                               |                                                                                  aCGTTATCCGTACAGTCGAGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGGCTGGCGTAATc                                                                        <  2:31945/90‑1 (MQ=255)    ttATCCGTACAGTCGAGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGGCTGGCGTAATcgcg                                                                     <  2:373715/90‑1 (MQ=255)         cGTACAGTCGAGCACCAGATCGTGTTCAGCAACCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGGCTGGCGTAATCGCGATAtg                                                                <  1:120534/90‑1 (MQ=255)                        cAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGGCTGGCGTAATCGCGATATGTGGGTTGATCCGCGt                                                 <  2:312680/90‑1 (MQ=255)                            tCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGGCTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAgg                                             >  2:157787/1‑90 (MQ=255)                                                      ttCTGCGTCATCCAGCAGTGCATTGGCTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCgg                   >  2:306383/1‑90 (MQ=255)                                                           cGTCATCCAGCAGTGCATTGGCTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGcc              <  1:149363/90‑1 (MQ=255)                                                                tCCAGCAGTGCATTGGCTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGAcc         <  1:160979/90‑1 (MQ=255)                                                                     cagTGCATTGGCTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGc    <  2:189870/90‑1 (MQ=255)                                                                       gTGCATTGGCTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCAt  >  1:357950/1‑90 (MQ=255)                                                                               |                                                                                  ACGTTATCCGTACAGTCGAGCACCAGATCGTGTTCAGCAATCAATGCTGCAAGTTCTGCGTCATCCAGCAGTGCATTGACTGGCGTAATCGCGATATGTGGGTTGATCCGCGTCAGGGCGTCACGGGCGGATTCCACCTTCGGTTGCCCGACCGTGGCAT  >  NZ_CP009273/860191‑860350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑