Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,194,248	A→G	D33G (GAC→GGC)	lit →	cell death peptidase Lit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,194,248	0	A	G	100.0%	65.6 / NA	20	D33G (GAC→GGC)	lit	cell death peptidase Lit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/8);  total (12/8)

TCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAG  >  NZ_CP009273/1194168‑1194334                                                                                 |                                                                                       tCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGGCAAAAAAAt                                                                               >  1:371751/1‑90 (MQ=255)                         atcaCCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAAcc                                                       >  2:67741/1‑90 (MQ=255)                               aTTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGttt                                                 >  2:225149/1‑90 (MQ=255)                               aTTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGttt                                                 >  1:222184/1‑90 (MQ=255)                               aTTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGttt                                                 >  1:314481/1‑90 (MQ=255)                                       ttGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATcc                                         >  1:184589/1‑90 (MQ=255)                                       ttGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATcc                                         >  1:393874/1‑90 (MQ=255)                                       ttGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATcc                                         >  1:218091/1‑90 (MQ=255)                                            ccAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTc                                    <  2:356676/90‑1 (MQ=255)                                              agagAAGGAGCAAGATCTTAAAACGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGCCAg                                  >  2:121369/1‑90 (MQ=255)                                                agaAGGAGCAAGATCTTAAAACGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGga                                <  2:184589/90‑1 (MQ=255)                                                agaAGGAGCAAGATCTTAAAACGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGga                                <  2:343806/90‑1 (MQ=255)                                                agaAGGAGCAAGATCTTAAAACGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGga                                <  2:393874/90‑1 (MQ=255)                                                    ggAGCAAGATCTTAAAACGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGgaagaa                            >  2:38563/1‑90 (MQ=255)                                                         aaGATCTTAAAACGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATgaga                       >  1:272199/1‑90 (MQ=255)                                                         aaGATCTTAAAACGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATgaga                       <  1:86711/90‑1 (MQ=255)                                                               ttAAAACGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGgaa                               >  1:361325/1‑76 (MQ=255)                                                               ttAAAACGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGgaa                               <  2:361325/76‑1 (MQ=255)                                                                  aaaCGATAGTTGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAAtt              <  1:189948/90‑1 (MQ=255)                                                                           ttGACGGCAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGaaa     <  2:222184/90‑1 (MQ=255)                                                                             gacggcAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATa   <  2:218091/90‑1 (MQ=255)                                                                              acggcAAAAAAATTAAAATTTCAGTTGTTAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAg  <  1:121369/90‑1 (MQ=255)                                                                                 |                                                                                       TCATCTTTTCTCAGCAATTAATTCATCACCATTTAAGATTGCACCAGAGAAGGAGCAAGATCTTAAAACGATAGTTGACGACAAAAAAATTATAATTTCAGTTGTGAGTGAACCTGGTTTTAATATCCGAGTCAGGAAGAATGAGAGTAATAATTCACATGAAATAG  >  NZ_CP009273/1194168‑1194334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑