Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,268,851	T→C	T69A (ACC→GCC)	ychN ←	DsrE/F sulfur relay family protein YchN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,268,851	0	T	C	100.0%	38.1 / NA	12	T69A (ACC→GCC)	ychN	DsrE/F sulfur relay family protein YchN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/6);  total (6/6)

TTTCCACCCCATCAATCAGAGGAAGTGTACTAATCCCGCGCCCGTCGGTACAGGTTTTGCACAATTTCACCGGTACATTCTGAGCGGTAAGGATCTCCAGCATTTGCTGAATGTTGTAGCCTTCCCCTGGTTTTTGCCCGCGCAACCCGGCTGTGACCGCATCAGAC  >  NZ_CP009273/1268764‑1268930                                                                                        |                                                                                tttCCACCCCATCAATCAGAGGAAGTGTACTAATCCCGCGCCCGTCGGTACAGGTTTTGCACAATTTCACCGGTACATTCTGAGCGGCaa                                                                               <  1:291312/90‑1 (MQ=255)   tCCACCCCATCAATCAGAGGAAGTGTACTAATCCCGCGCCCGTCGGTACAGGTTTTGCACAATTTCACCGGTACATTCTGAGCGGCAAgg                                                                             <  2:289971/90‑1 (MQ=255)                    agGAAGTGTACTAATCCCGCGCCCGTCGGTACAGGTTTTGCACAATTTCACCGGTACATTCTGAGCGGCAAGGATCTCCAGCATTTGCTg                                                            <  2:18401/90‑1 (MQ=255)                               tAATCCCGCGCCCGTCGGTACAGGTTTTGCACAATTTCACCGGTACATTCTGAGCGGCAAGGATCTCCAGCATTTGCTGAATGTTGTAGc                                                 >  1:178103/1‑90 (MQ=255)                                     cgcgCCCGTCGGTACAGGTTTTGCACAATTTCACCGGTACATTCTGAGCGGCAAGGATCTCCAGCATTTGCTGAATGTTGTAGCCTTccc                                           >  2:28734/1‑90 (MQ=255)                                                               aaTTTCACCGGTACATTCTGAGCGGCAAGGATCTCCAGCATTTGCTGAATGTTGTAGCCTTCCCCt                                         <  1:255428/66‑1 (MQ=255)                                                               aaTTTCACCGGTACATTCTGAGCGGCAAGGATCTCCAGCATTTGCTGAATGTTGTAGCCTTCCCCt                                         >  2:255428/1‑66 (MQ=255)                                                                        ggTACATTCTGAGCGGCAAGGATCTCCAGCATTTGCTGAATGTTGTAGCCTTCCCCTGGTTTTTGCCCGCGCAACCCGGCTGTGACCGCa        >  1:236275/1‑90 (MQ=255)                                                                          tACATTCTGAGCGGCAAGGATCTCCAGCATTTGCTGAATGTTGTAGCCTTCCCCTGGTTTTTGCCCGCGCAACCCGGCTGTGACCACATc      <  1:28734/90‑1 (MQ=255)                                                                              ttCTGAGCGGCAAGGATCTCCAGCATTTGCTGAATGTTGTAGCCTTCCCCTGGTTTTTGCCCGCGCAACCCGGCTGTGACCGGATCAGag  >  1:227393/1‑89 (MQ=255)                                                                                   aGCGGCAAGGATCTCCAGCATTTGCTGAATGTTGTAGCCTTCCCCTGGTTTTTGCCCGCGCAACCCGGCTGTGACCGCATCAg    >  1:257797/1‑83 (MQ=255)                                                                                   aGCGGCAAGGATCTCCAGCATTTGCTGAATGTTGTAGCCTTCCCCTGGTTTTTGCCCGCGCAACCCGGCTGTGACCGCATCAg    <  2:257797/83‑1 (MQ=255)                                                                                        |                                                                                TTTCCACCCCATCAATCAGAGGAAGTGTACTAATCCCGCGCCCGTCGGTACAGGTTTTGCACAATTTCACCGGTACATTCTGAGCGGTAAGGATCTCCAGCATTTGCTGAATGTTGTAGCCTTCCCCTGGTTTTTGCCCGCGCAACCCGGCTGTGACCGCATCAGAC  >  NZ_CP009273/1268764‑1268930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑