Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,394,163	G→A	L111L (CTG→TTG)	ogt ←	methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,394,163	0	G	A	100.0%	57.5 / NA	16	L111L (CTG→TTG)	ogt	methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/8);  total (8/8)

TACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC  >  NZ_CP009273/1394083‑1394240                                                                                 |                                                                              tACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTa                                                                      <  1:196389/90‑1 (MQ=255) tACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTa                                                                      <  2:282552/90‑1 (MQ=255)          gCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTAc                                                             >  1:58856/1‑90 (MQ=255)                    ttCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAg                                                   <  2:364362/90‑1 (MQ=255)                       gATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAgggg                                                >  2:120651/1‑90 (MQ=255)                            gTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGt                                           >  2:52877/1‑90 (MQ=255)                                              aCGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGAc                         >  1:250479/1‑90 (MQ=255)                                              aCGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGAc                         >  2:383389/1‑90 (MQ=255)                                                     gcgccAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCgcgc                  <  1:342709/90‑1 (MQ=255)                                                     gcgccAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCgcgc                  <  2:162511/90‑1 (MQ=255)                                                               ggCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCg        >  1:390618/1‑90 (MQ=255)                                                                gCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGt       >  2:380789/1‑90 (MQ=255)                                                                gCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGt       <  2:58856/90‑1 (MQ=255)                                                                  ccAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTcc     <  1:120651/90‑1 (MQ=255)                                                                    aaTTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTcccc   <  2:208528/90‑1 (MQ=255)                                                                     aTTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTccccc  >  2:161143/1‑90 (MQ=255)                                                                                 |                                                                              TACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCC  >  NZ_CP009273/1394083‑1394240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑