Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,558,439	A→G	Y518H (TAT→CAT)	dosP ←	oxygen‑sensing cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,558,439	0	A	G	100.0%	20.2 / NA	8	Y518H (TAT→CAT)	dosP	oxygen‑sensing cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/5);  total (3/5)

CTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAG  >  NZ_CP009273/1558358‑1558515                                                                                  |                                                                             caaaacCCAACCGCTTTTTTCCCTTTTCGTTCATCGCCGGGCTGAAAAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCcattg                                                                      <  2:134189/88‑1 (MQ=255)                  tttACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGgag                                                     <  1:356133/90‑1 (MQ=255)                        aTTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAg                                               >  2:307746/1‑90 (MQ=255)                               tCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCACCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgc                                        <  2:328430/90‑1 (MQ=255)                                       gggCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTa                                >  1:65626/1‑90 (MQ=255)                                                        gCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGAt               <  2:284152/90‑1 (MQ=255)                                                        caagTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGAt               <  2:259492/87‑1 (MQ=255)                                                                     ttCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAg  >  1:94220/1‑90 (MQ=255)                                                                                  |                                                                             CTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAG  >  NZ_CP009273/1558358‑1558515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑