Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	209,716	T→C	I300T (ATC→ACC)	tilS →	tRNA lysidine(34) synthetase TilS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	209,716	0	T	C	100.0%	18.7 / NA	7	I300T (ATC→ACC)	tilS	tRNA lysidine(34) synthetase TilS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/2);  total (5/2)

GGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGAC  >  NZ_CP009273/209647‑209791                                                                      |                                                                            ggATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAACCCGACGCTATCAGTCGCAAc                                                         <  1:259577/90‑1 (MQ=255)      tGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAACCCGACGCTATCAGTCGCAACTgtgg                                                    >  2:201974/1‑90 (MQ=255)                         cgcgGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAACCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCg                                 >  2:54696/1‑90 (MQ=255)                             ggAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAACCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCa                             <  2:77030/90‑1 (MQ=255)                                                  aCGTTTGGGCGCGTTTGAAACCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTg        >  1:247419/1‑90 (MQ=255)                                                   cGTTTGGGCGCGTTTGAAACCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTgg       >  1:70108/1‑90 (MQ=255)                                                        gggCGCGTTTGAAACCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGAc  >  1:302058/1‑90 (MQ=255)                                                                      |                                                                            GGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATCCGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATTGTGCCGTGGCAGAC  >  NZ_CP009273/209647‑209791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑