Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,717,498	G→C	G374A (GGC→GCC)	ydhK →	FUSC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,717,498	0	G	C	100.0%	26.2 / NA	9	G374A (GGC→GCC)	ydhK	FUSC family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (3/6);  total (3/6)

GGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCC  >  NZ_CP009273/1717414‑1717580                                                                                     |                                                                                   ggACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCAt                                                                               <  1:337558/90‑1 (MQ=255) ggACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCAt                                                                               <  1:370559/90‑1 (MQ=255)          gCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTg                                                                      <  2:13517/90‑1 (MQ=255)                                                    ggCTGCGTACATTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGgcag                            >  2:175972/1‑90 (MQ=255)                                                              aTTTTGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTg                  <  2:140693/90‑1 (MQ=255)                                                               ttttGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGc                                                                <  1:180708/43‑1 (MQ=255)                                                               ttttGTACGTTGATGATGATTGCCGCATGGAGTATTGCTTCGc                                                                >  2:180708/1‑43 (MQ=255)                                                                        ttGATGATGATTGCCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGtactctac        >  1:263572/1‑90 (MQ=255)                                                                              atgatTGCCGCATGGAGTATTGTTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTccgcc  <  1:38944/90‑1 (MQ=255)                                                                                     |                                                                                   GGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTAGCTGCGTACTCTACTCCGCC  >  NZ_CP009273/1717414‑1717580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑