| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,416,814 | T→C | K69E (AAA→GAA) | rpnB ← | recombination‑promoting nuclease RpnB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,416,814 | 0 | T | C | 100.0% | 44.3 / NA | 14 | K69E (AAA→GAA) | rpnB | recombination‑promoting nuclease RpnB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/2); total (12/2) | |||||||||||
GCATCATGCGAAAAGCCATTAATTCTTCCGGCTTACTTTGGTGCTCTATCACTACATAAATATAACCCACTCCCTCCTGCGTTTTCACAGACCACAAGAGGTCGGAATAATATTGCCGCAGGTCTTCATCAATAAAACTGTTTGGTTCCAGTTTAAGCGTCGTT > NZ_CP009273/2416730‑2416893 | gCATCATGCGAAAAGCCATTAATTCTTCCGGCTTACTTTGGTGCTCTATCACTACATAAATATAACCCACTCCCTCCTGCGTTTCCACAg > 2:73837/1‑90 (MQ=255) atGCGAAAAGCCATTAATTCTTCCGGCTTACTTTGGTGCTCTATCACTACATAAATATAACCCACTCCCTCCTGCGTTTCCACAGACcac > 2:249181/1‑90 (MQ=255) atGCG‑AAAGCCATTAATTCTTCCGGCTTACTTTGGTGCTCTATCACTACATAAATATAACCCACTCCCTCCTGCGTTTCCACAGACcaca > 2:281995/1‑90 (MQ=255) aGCCATTAATTCTTCCGGCTTACTTTGGTGCTCTATCACTACATAAATATAACCCACTCCCTCCTGCGTTTCCACAGACCACAAGAGGTc < 1:77135/90‑1 (MQ=255) ggCTTACTTTGGTGCTCTATCACTACATAAATATAACCCACTCCCTCCTGCGTTTCCACAGACCACAAGAGGTCGGAATAATATTGCCGc > 2:344051/1‑90 (MQ=255) ggCTTACTTTGGTGCTCTATCACTACATAAATATAACCCACTCCCTCCTGCGTTTCCACAGACCACAAGAGGTCGGAATAATATTGCCGc > 2:344052/1‑90 (MQ=255) aTAAATATAACCCACTCCCTCCTGCGTTTCCACAGACCACAAGAGGTCGGAATAATATTGCCGCAGGTCTTCATCAATAAAACTGTTTgg > 1:228570/1‑90 (MQ=255) aaCCCACTCCCTCCTGCGTTTCCACAGACCACAAGAGGTCGGAATAATATTGCCGCAGGTCTTCATCAATAAAACTGTTTGGTTCCAGtt > 2:75040/1‑90 (MQ=255) aCCCACTCCCTCCTGCGTTTCCACAGACCACAAGAGGTCGGAATAATATTGCCGCAGGTCTTCATCAATAAAACTGTTTGGTTCCAGttt > 1:343840/1‑90 (MQ=255) aCCCACTCCCTCCTGCGTTTCCACAGACCACAAGAGGTCGGAATAATATTGCCGCAGGTCTTCATCAATAAAACTGTTTGGTTCCAGttt > 1:45058/1‑90 (MQ=255) cACTCCCTCCTGCGTTTCCACAGACCACAAGAGGTCGGAATAATATTGCCGCAGGTCTTCATCAATAAAACTGTTTGGTTCCAGTTTAAg > 1:17031/1‑90 (MQ=255) aCTCCCTCCTGCGTTTCCACAGACCACAAGAGGTCGGAATAATATTGCCGCAGGTCTTCATCAATAAAACTGTTTGGTTCCAGTTTAAGc < 2:228570/90‑1 (MQ=255) ctccctccTGCGTTTCCACAGACCACAAGAGGTCGGAATAATATTGCCGCAGGTCTTCATCAATAAAACTGTTTGGTTCCAGTTTAAGcg > 2:84272/1‑90 (MQ=255) tcctGCGTTTCCACAGACCACAAGAGGTCGGAATAATATTGCCGCAGGTCTTCATCAATAAAACTGTTTGGTTCCAGTTTAAGCGTCGtt > 2:343995/1‑90 (MQ=255) | GCATCATGCGAAAAGCCATTAATTCTTCCGGCTTACTTTGGTGCTCTATCACTACATAAATATAACCCACTCCCTCCTGCGTTTTCACAGACCACAAGAGGTCGGAATAATATTGCCGCAGGTCTTCATCAATAAAACTGTTTGGTTCCAGTTTAAGCGTCGTT > NZ_CP009273/2416730‑2416893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |