Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,463,070	T→C	F154S (TTC→TCC)	BW25113_RS12270 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,463,070	0	T	C	100.0%	48.4 / NA	16	F154S (TTC→TCC)	BW25113_RS12270	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/7);  total (9/7)

CATTCTTTATTTTAGCTTTTCTTATGTGTTCTTCATCAACACTGTCAATGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAA  >  NZ_CP009273/2462983‑2463144                                                                                        |                                                                           cATTCTTTATTTTAGCTTTTCTTATGTGTTCTTCATCAACACTGTCAATGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTtccc                                                                          >  1:361459/1‑90 (MQ=255)             tAGCTTTTCTTATGTGTTCTTCATCAACACTGTCACTGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTCCCTTCCATTTTTgt                                                              >  1:381861/1‑89 (MQ=255)               gCTTTTCTTATGTGTTCTTCATCAACACTGTCAATGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTCCCTTCCATTTTTGgtg                                                            >  2:244702/1‑90 (MQ=255)               gCTTTTCTTATGTGTTCTTCATCAACACTGTCAATGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTCCCTTCCATTTTTGgtg                                                            <  2:220604/90‑1 (MQ=255)               gCTTTTCTTATGTGTTCTTCATCAACACTGTCAATGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTCCCTTCCATTTTTGgtg                                                            <  2:120137/90‑1 (MQ=255)                            gtTCTTCATCAACACTGTCAATGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTCCCTTCCATTTTTGGTGTATTCATTAACAt                                               >  2:347270/1‑90 (MQ=255)                                    tcaACACTGTCAATGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTCCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAAc                                       >  1:36278/1‑90 (MQ=255)                                            gTCAATGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTCCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATc                               >  2:239250/1‑90 (MQ=255)                                                      gCATCATTCTATCAAGAGCAAATAGTTATAATTTCCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTg                     >  2:63561/1‑90 (MQ=255)                                                        atcatTCTATCAAGAGCAAATAGTTATAATTTCCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTCTGCTTTTGCt                   <  2:361459/90‑1 (MQ=255)                                                              cTATCAAGAGCAAATAGTTATAATTTCCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTAttttt             >  1:326102/1‑90 (MQ=255)                                                                    agagCAAATAGTTATAATTTCCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGtt       <  2:14178/90‑1 (MQ=255)                                                                     gagCAAATAGTTATAATTTCCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTg      >  1:355677/1‑90 (MQ=255)                                                                       gCAAATAGTTATAATTTCCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCt    <  1:347270/90‑1 (MQ=255)                                                                         aaaTAGTTATAATTTCCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCtaa  <  1:244702/90‑1 (MQ=255)                                                                          aaTAGTTATAATTTCCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGc     >  2:188484/1‑86 (MQ=255)                                                                          aaTAGTTATAATTTCCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGc     <  1:188484/86‑1 (MQ=255)                                                                                        |                                                                           CATTCTTTATTTTAGCTTTTCTTATGTGTTCTTCATCAACACTGTCAATGTTTGCATCATTCTATCAAGAGCAAATAGTTATAATTTTCCTTCCATTTTTGGTGTATTCATTAACATGCAAAAACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAA  >  NZ_CP009273/2462983‑2463144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑