Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,443,321	(T)7→6	intergenic (+46/+12)	feaB → / ← tynA	phenylacetaldehyde dehydrogenase/primary‑amine oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,443,315	0	T	.	100.0%	117.0 / NA	28	intergenic (+40/+18)	feaB/tynA	phenylacetaldehyde dehydrogenase/primary‑amine oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (13/15);  total (13/15)

GGTGTGAAACTAAGTCG‑‑GTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAG  >  NZ_CP009273/1443241‑1443398                                                                             |                                                                                    ggTGTGAAACTAAGTCG‑‑GTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACa                                                                     <  2:704475/90‑1 (MQ=255)  gtgtGAAACTAAGTCG‑‑GTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACaa                                                                    <  2:299231/90‑1 (MQ=255)   tgtgAAACTAAGTCGGTGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACa                                                                     <  2:359140/90‑1 (MQ=38)                        gtgtACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAgcg                                                >  2:561/1‑90 (MQ=255)                              ggTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTa                                          <  2:687471/90‑1 (MQ=255)                                  ttAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGt                                      >  1:760015/1‑90 (MQ=255)                                  ttAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGt                                      >  1:991548/1‑90 (MQ=255)                                  ttAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGGGCCG‑TTTTTTGTCTGAAACAATCATTTATCTTTCTTCAGCGCCCCTAGCGt                                      >  1:322924/1‑90 (MQ=255)                                         ggTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGtt                               >  1:293865/1‑90 (MQ=255)                                         ggTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGtt                               <  1:384385/90‑1 (MQ=255)                                         ggTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGtt                               <  2:559131/90‑1 (MQ=255)                                         ggTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGtt                               >  1:705623/1‑90 (MQ=255)                                         ggTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGtt                               >  1:73326/1‑90 (MQ=255)                                             cGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGt                           >  1:241492/1‑90 (MQ=255)                                              gCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTc                          >  2:184182/1‑90 (MQ=255)                                                         aaaaaCGGCACCTGGTGCCG‑TTTTTTGTCTGGAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTcc               >  2:543191/1‑90 (MQ=255)                                                         aaaaaCGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACtt                                                              <  1:850755/43‑1 (MQ=255)                                                         aaaaaCGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACtt                                                              >  2:45229/1‑43 (MQ=255)                                                         aaaaaCGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACtt                                                              <  1:45229/43‑1 (MQ=255)                                                         aaaaaCGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACtt                                                              >  2:850755/1‑43 (MQ=255)                                                               ggCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGtt         <  2:293865/90‑1 (MQ=255)                                                               ggCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCAATGGtt         <  2:241492/90‑1 (MQ=255)                                                                gCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGttt        >  1:263154/1‑90 (MQ=255)                                                                 cACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTc       <  1:38968/90‑1 (MQ=255)                                                                 cACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTc       <  2:991548/90‑1 (MQ=255)                                                                      ggTGCCG‑TTTTTTGTCTGGAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTcagcag  <  1:543191/90‑1 (MQ=255)                                                                      ggTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTcatcag  <  1:184182/90‑1 (MQ=255)                                                                      ggTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTcagcag  <  1:70296/90‑1 (MQ=255)                                                                             |                                                                                    GGTGTGAAACTAAGTCG‑‑GTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAG  >  NZ_CP009273/1443241‑1443398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑