| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,473,944 | (G)6→7 | coding (550/1758 nt) | ynbC → | bifunctional alpha/beta hydrolase/class I SAM‑dependent methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,473,938 | 1 | . | G | 95.7% | 75.6 / ‑3.2 | 23 | coding (544/1758 nt) | ynbC | bifunctional alpha/beta hydrolase/class I SAM‑dependent methyltransferase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (13/9); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAA‑C‑GGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGT > NZ_CP009273/1473854‑1474016 | gcgcTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAA‑C‑ggggg > 1:404381/1‑90 (MQ=255) ggCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAA‑C‑gggggggcga > 2:681359/1‑86 (MQ=255) gTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTg < 1:681359/90‑1 (MQ=255) gTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGa < 1:71977/80‑1 (MQ=255) gTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGa > 2:71977/1‑80 (MQ=255) gTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTGATc > 2:301240/1‑90 (MQ=255) cTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTGATcac > 1:486542/1‑90 (MQ=255) aaTTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGc > 1:503942/1‑90 (MQ=255) aaTTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGc > 2:809155/1‑90 (MQ=255) aaTTCCTATGTGAAAGGACAGTTTTTGCCCCCCGATCGGGAATG‑GGGGGGCGAGCTTCAATAATGTTCCGCTGATGACACGGGCGATTGc < 2:293627/90‑1 (MQ=255) aaaGGACGCTATTTGACCCACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCtt > 2:636054/1‑90 (MQ=255) ggACGCTATTTGACCCACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCt > 1:735091/1‑90 (MQ=255) ggACGCTATTTGACCCACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCt < 2:363657/90‑1 (MQ=255) aCGCTATTTGACCCACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTGATc > 2:795696/1‑59 (MQ=255) aCGCTATTTGACCCACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTGATc < 1:795696/59‑1 (MQ=255) aCGCTATTTGACCCACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCg > 1:922169/1‑90 (MQ=255) gCTATTTGACCCACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGAt > 2:166903/1‑90 (MQ=255) tATTTGACCCACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACCCAGACGATTGCCGTTAATATCTTGCTCGAtct < 2:486542/90‑1 (MQ=255) gACCCACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCg < 1:964694/44‑1 (MQ=38) gACCCACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCg > 2:964694/1‑44 (MQ=38) cccACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACaaa < 1:994174/90‑1 (MQ=255) cccACGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACaaa < 2:735091/90‑1 (MQ=255) cGATCGGCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGt > 1:679274/1‑90 (MQ=255) ggCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTg < 1:933394/52‑1 (MQ=255) ggCAA‑CGGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTg > 2:933394/1‑52 (MQ=255) | GCGCTGGCGTTATGGCATCGTCTGCGTGGTCTGTTTTTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAA‑C‑GGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGT > NZ_CP009273/1473854‑1474016 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |