Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,255,828	+G	coding (1192/1209 nt)	tdcD ←	propionate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,255,825	1	.	G	93.5%	98.6 / 0.1	31	coding (1195/1209 nt)	tdcD	propionate kinase
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base G (21/8);  total (23/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGTACAGCTTATCGCTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGC‑GGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACA  >  NZ_CP009273/3255739‑3255907                                                                                        |                                                                                    ggCGTACAGCTTATCGCTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGC‑ggg                                                                                  <  1:229217/90‑1 (MQ=255)   cGTACAGCTTATCGCTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGC‑ggggc                                                                                <  1:1049469/90‑3 (MQ=255)   cGTACAGCTTATCGCTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGC‑ggggc                                                                                <  2:452663/90‑3 (MQ=255)       cAGCTTATCGCTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGG‑CGCGt                                                                            <  1:403441/90‑1 (MQ=255)            tATCGCTGGTATCAATATCTACCTTCATGAAAAATAATCTATCTACAATACTTCAACTAAATTATGAAAATTCTGC‑GGGCCGCGTTAACt                                                                       >  2:788343/1‑90 (MQ=255)                 cTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGcc                                                                  >  2:995997/1‑90 (MQ=255)                  tGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCt                                                                 <  1:397641/90‑1 (MQ=255)                   ggTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTa                                                                >  2:842226/1‑90 (MQ=255)                   ggTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGC‑GGGTCGCGTTAACTTTGCCTa                                                                >  1:128462/1‑90 (MQ=255)                         aaTATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAaatgaa                                                          <  1:954588/90‑1 (MQ=255)                                    tCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCaaa                                               >  2:76721/1‑90 (MQ=255)                                    tCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGC‑ggg                                                                                  >  2:931420/1‑55 (MQ=255)                                    tCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGC‑ggg                                                                                  <  1:931420/55‑1 (MQ=255)                                     cATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAg                                              >  2:316522/1‑90 (MQ=255)                                       tGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCa                                            <  1:316522/90‑1 (MQ=255)                                                    ctctACAATACTTCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTttctt                               >  1:1051377/1‑90 (MQ=255)                                                     tctACAATACTTCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTttcttc                              >  1:138855/1‑90 (MQ=255)                                                           aTACTTCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGt                        >  2:434293/1‑90 (MQ=255)                                                            tACTTCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTc                       <  1:130719/90‑1 (MQ=255)                                                              cTTCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCgg                     >  1:855107/1‑90 (MQ=255)                                                              cTTCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCgg                     >  2:834829/1‑90 (MQ=255)                                                              cTTCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCgg                     >  2:831502/1‑90 (MQ=255)                                                                tCAACTAAATTATGCAAATTCTTCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGaa                   >  2:733313/1‑90 (MQ=255)                                                                tCAACTAAATTATGCAAATTCTTCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGaa                   >  1:320086/1‑90 (MQ=255)                                                                tCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGaa                   >  1:63231/1‑90 (MQ=255)                                                                tCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGaa                   >  2:110635/1‑90 (MQ=255)                                                                tCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGaa                   >  1:390855/1‑90 (MQ=255)                                                                tCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGaa                   >  1:376583/1‑90 (MQ=255)                                                                tCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGaa                   >  1:187504/1‑90 (MQ=255)                                                                tCAACTAAATTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGaa                   >  2:780770/1‑90 (MQ=255)                                                                tCAACTAAATTATGCAAATTCTGCGGGG‑AGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGaa                   >  2:70202/1‑90 (MQ=255)                                                                      aaattatgcaaattatgcgGGG‑CGCGGGAACGTTGCGTAAATGAATTGCATCCAAAGAAATCGTTTTTTCATCTTTAGTTGGAATacagg             >  2:55029/1‑86 (MQ=255)                                                                       aaTTATGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGc            >  2:735089/1‑90 (MQ=255)                                                                         ttATGCAAATTCTGCGGGG‑AGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGcac          <  1:70202/90‑1 (MQ=255)                                                                           aTGCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACaa        >  1:826374/1‑90 (MQ=255)                                                                             gCAAATTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAt      <  1:213645/90‑1 (MQ=255)                                                                                 aTTCTGCGGGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGaca  <  1:780770/90‑1 (MQ=255)                                                                                        |                                                                                    GGCGTACAGCTTATCGCTGGTATCAATATCTACCTTCATGAAAAATAATCTCTCTACAATACTTCAACTAAATTATGCAAATTCTGC‑GGG‑CGCGTTAACTTTGCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACA  >  NZ_CP009273/3255739‑3255907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑