New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NZ_CP009273	= 3609927	60 (1.700)	3 (0.090)	3/148	2.5	6.2%	coding (386/834 nt)	nikC	nickel ABC transporter permease subunit NikC
	?	NZ_CP009273	= 3610020	37 (1.160)					coding (479/834 nt)	nikC	nickel ABC transporter permease subunit NikC
Rejected: Frequency below/above cutoff threshold.

TTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP009273/3609837‑3609927 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gttggcgtAGTGAAATCACCAGGCTGCGCACCATGCGT  <  NZ_CP009273/3610020‑3609991                                                                                                                           TTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCG                                 <  1:51671/90‑1 TTGGAGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCG                                 <  2:366866/90‑1    GCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTAG                              <  1:292025/90‑1                                  TCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCG                                 <  1:288179/57‑1                                  TCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCG                                 >  2:288179/1‑57                                                 TGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTAGTGAAATCACCAGGCTGCGCACCATGCGT  >  1:850290/1‑73                                                 TGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTAGTGAAATCACCAGGCTGCGCACCATGCGT  <  2:850290/73‑1                                                      TTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCG                                 >  1:234528/1‑37                                                      TTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCG                                 <  2:234528/37‑1                                                                                                                           TTGGCGGGCGCGTTGATCAGGCCACCATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP009273/3609837‑3609927 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gttggcgtAGTGAAATCACCAGGCTGCGCACCATGCGT  <  NZ_CP009273/3610020‑3609991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.