Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,558,3730CT52.9% 6.4 / 17.4 17E540K (GAG→AAG) dosPoxygen‑sensing cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (5/3);  new base T (0/9);  total (5/12)
Fisher's exact test for biased strand distribution p-value = 9.05e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGA  >  NZ_CP009273/1558290‑1558455
                                                                                   |                                                                                   
tGCGAACATTTGCGGCTGGTAACCCAATTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCTAAAACCAAACGCTCTTTTAc                                                                               <  2:416795/90‑1 (MQ=255)
  cGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCTAAAACCAAACGCTCTTTTACCa                                                                             >  2:62478/1‑90 (MQ=255)
          tGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTc                                                                     <  1:506564/90‑1 (MQ=255)
                                   ggTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCTAAAACCAAACGCTCTTTTACCATCTCGTTCATCGCCGGGCTGAAGAACTGCCAGc                                           >  1:799721/1‑90 (MQ=255)
                                       aTTGCTAATCGTTTTTTCCGGCGCTGCGCCAAAACCCAACCCCTCTTTTCCCTTTTCGTTCATCGCGGGGCTGAAAACCTGCCAGCCGtt                                       <  2:44085/90‑1 (MQ=255)
                                       aTTGCTAATCGCTTCTTTCAGCGCTGCGCCTAAAACCAAACGCTTTTTTACCATTTCTTTCATCGCCGGGCTGAAGAACTGCCAGCCGtt                                       <  1:327318/90‑1 (MQ=255)
                                                      tttCAGCGCGGGCCCAAAAACCAACCCCTTTTTTCCTTTTTCTTTCACCGCCGGGCGGAAAAACGGCAGGCCGTTACCGCCATTCTTGCg                        <  2:542616/90‑1 (MQ=255)
                                                          aGCGCTGCGCCTAAACCCAAACGCTTTTTTCCCATTTCTTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAata                    <  1:450067/90‑1 (MQ=255)
                                                           ccgcgGCGCATAAACCCACACTCTTTTTTCCCTTTTCGTCCACCCCGGGCCTAAAAACTTGCCAGCCGTTCCCGCCATTCTTGCGAatat                   <  2:667691/89‑1 (MQ=255)
                                                               tGCGCCTAAAACCAACCGCTTTTTTCCCATTTCTTTCATCGCGGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATc               <  1:10913/90‑1 (MQ=255)
                                                               tGCCCCAAAAACAAAACGCTTTTTTCCCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATc               <  1:62478/90‑1 (MQ=255)
                                                                gcgcCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATcc              >  2:162420/1‑90 (MQ=255)
                                                                         aCCAAACGCTTTTTTCCCATTTCGTTCATCGCCGGGCTAAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCcattgcattg     <  1:204015/90‑1 (MQ=255)
                                                                           cAACCGCTTTTTTCCCATTTCTTTCACCGCCGGGTTAAAAACCTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATtgtg   <  2:913304/90‑1 (MQ=255)
                                                                           cAACCGCTTTTTACCCTTTTCTTTCACCCCGGGGCTGAAGAACTGCCAGCCGTTCCCGCCATTCTTGCGAATATAATCCATTGCATtgtg   <  2:601231/90‑1 (MQ=255)
                                                                            aaaCGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGa  >  2:50747/1‑90 (MQ=255)
                                                                            aaaCGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGa  >  1:700935/1‑90 (MQ=255)
                                                                                   |                                                                                   
TGCGAAGATTTGCGGCTGGTAAACCAGTTTCAGTTGGTTATTGCTAATCGCTTCTTTCAGCGCTGCGCCTAAAACCAAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGA  >  NZ_CP009273/1558290‑1558455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: