| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,698,298 | 0 | G | T | 57.9% | 13.1 / 28.0 | 19 | H90N (CAT→AAT) | ydgJ | oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (8/0); new base T (0/11); total (8/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGG > NZ_CP009273/1698212‑1698379 | cagCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTTATCAACGACCACATGttt > 1:1029134/1‑90 (MQ=255) ccccAGGCTTTTTGCCATCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTTATCAACGACCACATGTTTACCCGCCTc > 1:987004/1‑90 (MQ=255) gttttttGCCGGCCCACCCAGCTCCCGCGTTTGTGACGGTGCCAGGGTAAGGGT‑TTTATCAACGCCCCCTTTTTTCCCCGCCTCAAgcgc < 1:408695/88‑1 (MQ=255) gttttttCCCGGCGCTCCCACCTCCCGCGTTTGTGACAGTGTCAGGGTAAGGGT‑TTTACAACCGCCCACTTTTTTACCCGCCTCAAgcgc < 1:449407/88‑1 (MQ=255) tttGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCt > 2:934752/1‑90 (MQ=255) aGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCt > 2:816094/1‑90 (MQ=255) aTCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACgg > 2:633607/1‑90 (MQ=255) cgcctctCGCGTTTGTGACAGTGTCAGGGTAAGGGG‑TTTATCACCGCCCACTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGaa < 1:403237/87‑1 (MQ=255) ttGTGACAGTGCCCGGGTAAGGGT‑TTTAACAACCCCCACTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGtt < 2:987004/90‑1 (MQ=255) tgtgACGGTGTCAGGGAAAGGGT‑TTTACAAACGACCACTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTg < 1:197230/90‑1 (MQ=255) tgggACGGTGTCAGGGAAAGGGT‑TTTACCAACGCCCAATTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTg < 1:591447/87‑1 (MQ=255) tgggACAGTGCCGGGGAAAGGGT‑TTTAACAACGACCATTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTg < 2:993081/87‑1 (MQ=255) cgACGGTGCCACGATAAGGGT‑TTTACCAACGTCCATATTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTggg < 1:633607/89‑1 (MQ=255) gtgtCACGGTAAAGGG‑TTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAg > 1:590897/1‑90 (MQ=255) ggaaaaGGT‑TTTACAAACGACCATTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGAc < 2:1029134/87‑1 (MQ=255) aaGGGTTTTACCAACAACCATTTTTTTCCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAAt < 2:590897/90‑1 (MQ=255) tttATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTc > 1:340901/1‑90 (MQ=255) tATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTa > 1:914499/1‑90 (MQ=255) ccccAAATTTTTCCCCGCCTCAAGCGCCGCTTTGGATAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTggg < 1:402787/88‑1 (MQ=255) | CAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGG‑TTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGG > NZ_CP009273/1698212‑1698379 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |