Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	12,125	A→C	intergenic (‑339/‑38)	yaaI ← / → dnaK	DUF2541 family protein/molecular chaperone DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	12,125	0	A	C	100.0%	149.2 / NA	41	intergenic (‑339/‑38)	yaaI/dnaK	DUF2541 family protein/molecular chaperone DnaK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/27);  total (14/27)

TTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAG  >  NZ_CP009273/12039‑12211                                                                                       |                                                                                       ttAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCaccacc                                                                                     <  1:703149/90‑1 (MQ=255) ttAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCaccacc                                                                                     <  1:439053/90‑1 (MQ=255)      agtCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACatg                                                                                <  2:111976/90‑1 (MQ=255)        tCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCCCCACCACatgat                                                                              <  1:257406/90‑1 (MQ=255)        tCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACatgat                                                                              >  1:675413/1‑90 (MQ=255)        tCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACatgat                                                                              >  2:474011/1‑90 (MQ=255)         cAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACatgatg                                                                             >  1:209833/1‑90 (MQ=255)          aaCCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACAtgatga                                                                            <  2:28857/90‑1 (MQ=255)            ccGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGAcc                                                                          <  2:902305/90‑1 (MQ=255)            ccGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGAcc                                                                          <  1:666213/90‑1 (MQ=255)            ccGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGAcc                                                                          <  2:10957/90‑1 (MQ=255)              gCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTTAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGa                                                                        >  2:488363/1‑90 (MQ=255)               cAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGaa                                                                       <  2:35462/90‑1 (MQ=255)               cAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGaa                                                                       <  2:13870/90‑1 (MQ=255)                 gtgagtgaGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAata                                                                     >  2:577895/1‑90 (MQ=255)                 gtgagtgaGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAata                                                                     >  2:817894/1‑90 (MQ=255)                  tgagtgagTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAatat                                                                    <  2:368661/90‑1 (MQ=255)                  tgagtgagTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAatat                                                                    <  2:913115/90‑1 (MQ=255)                     gtgagtCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAAtatata                                                                 >  1:429876/1‑90 (MQ=255)                     gtgagtCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAAACGTTTCGCCCCTATTACAGACGCACCACCACATGATGACCGAAtatata                                                                 >  1:816577/1‑90 (MQ=255)                         gtCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTgg                                                             >  2:989025/1‑90 (MQ=255)                           cTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGgag                                                           <  1:466388/90‑1 (MQ=255)                           cTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGgag                                                           <  1:577895/90‑1 (MQ=255)                                   aaaTGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCaccacc                                                                                     >  1:222740/1‑56 (MQ=255)                                   aaaTGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCaccacc                                                                                     <  2:222740/56‑1 (MQ=255)                                    aaTGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGa                                                  <  1:790506/90‑1 (MQ=255)                                     aTGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGAt                                                 >  2:553498/1‑90 (MQ=255)                                         aaTTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGt                                             <  1:989025/90‑1 (MQ=255)                                             gggCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTaaaa                                         <  2:698402/90‑1 (MQ=255)                                                  gTTGAAACCAGACGTTTCGCCCCTATTACAGACTCGCCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAAtt                                    <  1:366811/90‑1 (MQ=255)                                                  gTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAAtt                                    <  1:474011/90‑1 (MQ=255)                                                  gTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAAtt                                    <  1:817894/90‑1 (MQ=255)                                                       aaCCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAAtt                                    >  2:37682/1‑85 (MQ=255)                                                       aaCCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAAtt                                    <  1:37682/85‑1 (MQ=255)                                                       aaCCAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTAt                               <  2:205056/90‑1 (MQ=255)                                                         ccAGACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCg                             <  1:575688/90‑1 (MQ=255)                                                            gACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCg                             <  2:319321/87‑1 (MQ=255)                                                            gACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCg                             >  1:319321/1‑87 (MQ=255)                                                            gACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGAcc                          <  1:553498/90‑1 (MQ=255)                                                            gACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGAcc                          >  1:685863/1‑90 (MQ=255)                                                            gACGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGAcc                          <  1:372390/90‑1 (MQ=255)                                                              cGTTTCGCCCCTATTACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTg                        <  1:371922/90‑1 (MQ=255)                                                                        cTATTACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCa              <  2:106396/90‑1 (MQ=255)                                                                            tACAGACTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAActc          <  1:708162/90‑1 (MQ=255)                                                                                  cTCACCACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTCgtgt    >  1:1038069/1‑90 (MQ=255)                                                                                    caccaccacATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAActct         <  2:1055714/83‑1 (MQ=255)                                                                                    caccaccacATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAg  <  2:430943/90‑1 (MQ=255)                                                                                       |                                                                                       TTAGTAGTCAACCGCAGTGAGTGAGTCTGCAAAAAAATGAAATTGGGCAGTTGAAACCAGACGTTTCGCCCCTATTACAGACTCACAACCACATGATGACCGAATATATAGTGGAGACGTTTAGATGGGTAAAATAATTGGTATCGACCTGGGTACTACCAACTCTTGTGTAG  >  NZ_CP009273/12039‑12211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑