Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	450,563	G→A	intergenic (+318/‑26)	bolA → / → tig	transcriptional regulator BolA/trigger factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	450,563	0	G	A	100.0%	123.0 / NA	45	intergenic (+318/‑26)	bolA/tig	transcriptional regulator BolA/trigger factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/19);  total (26/19)

CAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCT  >  NZ_CP009273/450484‑450648                                                                                |                                                                                      cAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGAtt                                                                             >  2:962107/1‑90 (MQ=255)      aaTGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTg                                                                        >  1:357872/1‑90 (MQ=255)         gtgATTGCTTATCAGAACCCCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAgg                                                                     <  2:357872/90‑1 (MQ=255)         gtgATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAgg                                                                     <  1:962107/90‑1 (MQ=255)           gattgattATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTa                                                                   >  2:899146/1‑90 (MQ=255)               gattATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACaa                                                               >  1:110261/1‑90 (MQ=255)               gattATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACaa                                                               >  1:213074/1‑90 (MQ=255)               gattATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACaa                                                               >  1:287173/1‑90 (MQ=255)               gattATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACaa                                                               >  1:711609/1‑90 (MQ=255)               gattATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTATGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACaa                                                               <  2:304608/90‑1 (MQ=255)                   ataAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGAtttttt                                                                         >  1:73652/1‑76 (MQ=255)                   ataAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGAtttttt                                                                         <  2:73652/76‑1 (MQ=255)                     aaGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCa                                                         <  2:116788/90‑1 (MQ=255)                         aCATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGtt                                                     >  2:490061/1‑90 (MQ=255)                         aCATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGtt                                                     >  2:392543/1‑90 (MQ=255)                         aCATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGtt                                                     >  1:766587/1‑90 (MQ=255)                          cATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCaa                                                        <  2:55853/86‑1 (MQ=255)                          cATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCaa                                                        >  1:55853/1‑86 (MQ=255)                          cATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGttt                                                    <  2:655904/90‑1 (MQ=255)                          cATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGttt                                                    >  1:475476/1‑90 (MQ=255)                            tCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCa                                                  >  2:35078/1‑90 (MQ=255)                                ggTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTg                                              <  1:425029/90‑1 (MQ=255)                                 gTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGa                                             >  2:888844/1‑90 (MQ=255)                                 gTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGa                                             >  1:656090/1‑90 (MQ=255)                                  ttCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGaa                                            <  1:181947/90‑1 (MQ=255)                                    ccGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAAc                                          <  1:35078/90‑1 (MQ=255)                                     cgcgAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAAcc                                         <  1:786967/90‑1 (MQ=255)                                           gCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCaa                                   <  1:899146/90‑1 (MQ=255)                                           gCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCaa                                   <  1:551539/90‑1 (MQ=255)                                                     tgtgCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGgcc                         >  1:493509/1‑90 (MQ=255)                                                     tgtgCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGgcc                         >  2:1044447/1‑90 (MQ=255)                                                     tgtgCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGgcc                         >  2:882488/1‑90 (MQ=255)                                                     tgtgCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGgcc                         >  2:480270/1‑90 (MQ=255)                                                       tgCTTGCGGGGTGAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGgccgc                       <  1:956170/90‑1 (MQ=255)                                                       tgCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGgccgc                       >  2:71720/1‑90 (MQ=255)                                                       tgCTTGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGgccgc                       <  2:493509/90‑1 (MQ=255)                                                          ttGCGGGGTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCgt                    >  1:153780/1‑90 (MQ=255)                                                                 gTAAGAGTTGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGa             <  2:475476/90‑1 (MQ=255)                                                                        ttGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTAt      <  2:287173/90‑1 (MQ=255)                                                                         tGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGgccg                        <  1:980055/71‑1 (MQ=255)                                                                         tGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGgccg                        >  2:980055/1‑71 (MQ=255)                                                                         tGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATc     >  1:661581/1‑90 (MQ=255)                                                                         tGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATc     >  1:43242/1‑90 (MQ=255)                                                                         tGACCGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATc     >  1:243842/1‑90 (MQ=255)                                                                            ccGAACACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCgct  <  1:370069/90‑1 (MQ=255)                                                                                |                                                                                      CAGGGAATGTGATTGATTATAAGAACATCCCGGTTCCGCGAAGCCAACAACCTGTGCTTGCGGGGTAAGAGTTGACCGAGCACTGTGATTTTTTGAGGTAACAAGATGCAAGTTTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCT  >  NZ_CP009273/450484‑450648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑