Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	454,444	A→G	E34G (GAA→GGA)	lon →	endopeptidase La

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	454,444	0	A	G	100.0%	131.9 / NA	37	E34G (GAA→GGA)	lon	endopeptidase La
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/16);  total (21/16)

GTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGAAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGA  >  NZ_CP009273/454357‑454528                                                                                        |                                                                                     gTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGgaa                                                                                    <  1:539974/90‑1 (MQ=255)  ttCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCTGGCGGGgaaa                                                                                   >  1:176470/1‑90 (MQ=255)          gCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATcc                                                                           <  2:141001/90‑1 (MQ=255)           cATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCg                                                                          >  1:1009697/1‑90 (MQ=255)            aTTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGt                                                                         <  2:567307/90‑1 (MQ=255)                     cccGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGaa                                                                <  1:252436/90‑1 (MQ=255)                       cGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAgc                                                              <  2:510991/90‑1 (MQ=255)                       cGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAgc                                                              <  1:450219/90‑1 (MQ=255)                             gCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGaa                                                                <  1:555398/82‑1 (MQ=255)                             gCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTATGGaa                                                                >  2:555398/1‑82 (MQ=255)                               cGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGATgg                                                      >  2:18834/1‑90 (MQ=255)                                 cTGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGAc                                                    <  2:282461/90‑1 (MQ=255)                                  tGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGAcc                                                   <  1:202616/90‑1 (MQ=255)                                  tGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGAcc                                                   >  1:785811/1‑90 (MQ=255)                                  tGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGAcc                                                   >  2:888573/1‑90 (MQ=255)                                   gcgcGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCTTTGTCTGGAAGCGGCGATGGACCa                                                  >  2:740947/1‑90 (MQ=255)                                                  ttATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGgaa                                                                                    <  1:707788/41‑1 (MQ=255)                                                  ttATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGgaa                                                                                    >  2:707788/1‑41 (MQ=255)                                                  ttATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAgc                                                              >  2:999840/1‑63 (MQ=255)                                                  ttATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAgc                                                              <  1:999840/63‑1 (MQ=255)                                                  ttATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGAt                                                        >  1:989487/1‑69 (MQ=255)                                                  ttATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGAt                                                        <  2:989487/69‑1 (MQ=255)                                                  ttATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATaaa                                           <  1:617982/82‑1 (MQ=255)                                                  ttATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATaaa                                           >  2:617982/1‑82 (MQ=255)                                                  ttATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTAt                                   >  1:640050/1‑90 (MQ=255)                                                    aTCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGt                                                                         >  2:910248/1‑50 (MQ=255)                                                    aTCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGt                                                                         <  1:910248/50‑1 (MQ=255)                                                                  aTCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGGTCGCGCagaaa                   >  1:506519/1‑90 (MQ=255)                                                                   tCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGGTcg                           <  1:157400/81‑1 (MQ=255)                                                                   tCCCCTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGGTcg                           >  2:157400/1‑81 (MQ=255)                                                                       cTTATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGc              >  2:305891/1‑90 (MQ=255)                                                                        ttATTTGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCt             <  1:948788/90‑1 (MQ=255)                                                                             tGTCGGTCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAAc        >  1:984842/1‑90 (MQ=255)                                                                             tGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAAc        >  2:356041/1‑90 (MQ=255)                                                                             tGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAAc        >  1:404481/1‑90 (MQ=255)                                                                             tGTCGGGCGGGGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGGTCGAGCAGAAAGAAGCTTCAAc        <  2:640050/90‑1 (MQ=255)                                                                               tCGGGCGGGGAAAAACTATCCGTTGTCTGGAAGCGGCGTTGGACCATGATAAAAAAATTATGCTGGCCGCGCAGAAAGAAGCTTCAACgg      >  2:833537/1‑90 (MQ=255)                                                                                cgggcgggGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGa     >  2:859888/1‑90 (MQ=255)                                                                                 gggcgggGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAGCGGAt    >  2:1067166/1‑90 (MQ=255)                                                                                 gggcgggGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGAt    <  2:619032/90‑1 (MQ=255)                                                                                   gcgggGAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGa  >  1:347372/1‑90 (MQ=255)                                                                                        |                                                                                     GTTCTGAACGCATTGAAATCCCCGTATTGCCGCTGCGCGATGTGGTGGTTTATCCGCACATGGTCATCCCCTTATTTGTCGGGCGGGAAAAATCTATCCGTTGTCTGGAAGCGGCGATGGACCATGATAAAAAAATTATGCTGGTCGCGCAGAAAGAAGCTTCAACGGATGA  >  NZ_CP009273/454357‑454528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑