| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 500,156 | A→G | intergenic (‑4/‑214) | fsr ← / → ushA | fosmidomycin MFS transporter/bifunctional UDP‑sugar hydrolase/5'‑nucleotidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 500,156 | 0 | A | G | 87.5% | 39.5 / ‑0.7 | 16 | intergenic (‑4/‑214) | fsr/ushA | fosmidomycin MFS transporter/bifunctional UDP‑sugar hydrolase/5'‑nucleotidase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (8/6); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.40e-01 | |||||||||||
ACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAA > NZ_CP009273/500069‑500241 | aCCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGGTGTTCACTCATTGCCATAGGgaa > 2:324816/1‑90 (MQ=255) cAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGGAAGTCACTTTTTc > 1:535215/1‑90 (MQ=255) gcGGGCTTTGGTCGTCGCCGCGGCCGCCCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGGAAGTCACTTTTTCAGGGTTGCGa < 2:535215/89‑1 (MQ=255) cTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGGAAGTCACTTTTTCAGGGTTGCGATGTaaa > 2:170904/1‑90 (MQ=255) cTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGGAAGTCACTTTTTCAGGGTTGCGATGTaaa > 2:133992/1‑90 (MQ=255) ggTCGTTGCCGCCCCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGGAAGTCACTTTTTCAGGGTTGCGATGTAAAGaa < 1:932079/90‑1 (MQ=255) gCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGGAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTAtt > 1:149642/1‑90 (MQ=255) caGGCTGGGGTTGTTCACTCATTGCCATAGGGAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGa < 1:543910/90‑1 (MQ=255) caGGCTGGGGTTGTTCACTCATTGCCATAGGGAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGa < 1:483996/90‑1 (MQ=255) ggCTGGGGTTGTTCACTCATTGCCATAGGGAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACt < 1:393434/90‑1 (MQ=255) gggTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACAt < 1:873486/90‑1 (MQ=255) ggTTGTTCACTCATTGCCATAGGGAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATa < 1:133992/90‑1 (MQ=255) tgttCACTCATTGCCATAGGGAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCt > 1:441567/1‑90 (MQ=255) gttCACTCATTGCCATAGGGAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTg > 1:620371/1‑90 (MQ=255) gttCACTCATTGCCATAGGGAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTg > 1:781437/1‑90 (MQ=255) gttCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTg > 1:342563/1‑90 (MQ=255) tAGGGAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTgaa > 2:44411/1‑90 (MQ=255) | ACCTAAAATACCAAACGATGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAA > NZ_CP009273/500069‑500241 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |