Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	656,002	T→C	E278G (GAA→GGA)	ybeF ←	YbeF family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	656,002	0	T	C	100.0%	72.1 / NA	21	E278G (GAA→GGA)	ybeF	YbeF family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/9);  total (12/9)

GCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCAACCAGTGCAGCGAT  >  NZ_CP009273/655916‑656087                                                                                       |                                                                                      gCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATATAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTCCCa                                                                                    >  1:874771/1‑90 (MQ=255)    ggATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTCCCAGCg                                                                                 >  2:744361/1‑90 (MQ=255)         ggATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTCCCAGCGGCcag                                                                            >  2:325389/1‑90 (MQ=255)         ggATCACGCAGGATGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTCCCAGCGGCcag                                                                            >  2:556779/1‑90 (MQ=255)           aTCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGTCAGTTTTCCCAGCGGCcagca                                                                          <  2:700831/90‑1 (MQ=255)              aCGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTCCCAGCGGCCAGCAGCg                                                                       >  1:864195/1‑90 (MQ=255)                gCAGGATGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTCCCAGCGGCCAGCAGCGGc                                                                     <  1:556779/90‑1 (MQ=255)                     cTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTCCCAGCGGCCAGCAGCGGCTAAAc                                                                <  2:685284/90‑1 (MQ=255)                     cTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTCCCAGCGGCCAGCAGCGGCTAAAc                                                                <  2:324842/90‑1 (MQ=255)                             gttgtAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTCCCAGCGGCCAGCAGCGGCTAAACAGGTtata                                                        >  1:292772/1‑90 (MQ=255)                                gtAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTCCCAGCGGCCAGCAGCGGCTAAACAGGTTATaaaa                                                     <  2:864195/90‑1 (MQ=255)                                     ggATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTCCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGc                                                <  1:427178/90‑1 (MQ=255)                                     ggATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTCCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGc                                                >  2:348428/1‑90 (MQ=255)                                               aTCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTCCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGaattat                                      >  2:251762/1‑90 (MQ=255)                                               aTCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTCCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGaataat                                      <  1:130480/90‑1 (MQ=255)                                                   aTTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTCCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAAGCGCt                                  >  2:591449/1‑90 (MQ=255)                                                      tGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTCCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAAc                               >  1:626092/1‑90 (MQ=255)                                                           ctcATTTAAGGACGGAAAAGGCAGTTTTCCCAGCGGCCAGCAGCGGCTAAACAGGTTAT‑‑AAACGGCTGGGAATAATCGCTAACATGTCAc                        <  1:208459/90‑1 (MQ=255)                                                                       cGGAAAAGGCAGTTTTCCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCAAc              >  1:872669/1‑90 (MQ=255)                                                                        ggAAAAGGCAGTTTTCCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCAAcc             <  2:172530/90‑1 (MQ=255)                                                                                 aGTTTTCCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCAACCAGTGCAGCg    >  1:755037/1‑90 (MQ=255)                                                                                   ttttcCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCAACCAGTGCAGCGAt  <  1:744361/90‑1 (MQ=255)                                                                                       |                                                                                      GCAGGATGGGATCACGCAGGCTGAATTTGTTGTAGTGGATAGAGAAATCTATTTGCTCCTCATTTAAGGACGGAAAAGGCAGTTTTTCCAGCGGCCAGCAGCGGCTAAACAGGTTATAAAAACGGCTGGGAATAATCGCTAACATGTCACTGTTGGCAACCAGTGCAGCGAT  >  NZ_CP009273/655916‑656087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑