Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	44,049	A→G	I288V (ATC→GTC)	fixB →	electron transfer flavoprotein subunit alpha/FixB family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	44,049	0	A	G	100.0%	127.9 / NA	36	I288V (ATC→GTC)	fixB	electron transfer flavoprotein subunit alpha/FixB family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/21);  total (15/21)

GTGGGGATCTCCGGGCAGATCCAGCACATGGTTGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGATCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACT  >  NZ_CP009273/43962‑44135                                                                                        |                                                                                       gTGGGGATCTCCGGGCAGATCCAGCACATGGTTGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTc                                                                                      >  1:247364/1‑90 (MQ=255) gTGGGGATCTCCGGGCAGATCCAGCACATGGTTGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTc                                                                                      >  1:412548/1‑90 (MQ=255)  tGGGGATCTCCGGGCAGATCCAGCACATGGTTGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCt                                                                                     <  1:100516/90‑1 (MQ=255)     ggATCTCCGGGCAGATCCAGCACATGGTTGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTcc                                                                                  <  1:973864/90‑1 (MQ=255)      gATCTCCGGGCAGATCCAGCACATGGTTGGCACTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCa                                                                                 <  1:306285/90‑1 (MQ=255)         ctcCGGGCAGATCCAGCACATGGTTGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTa                                                                              <  1:658088/90‑1 (MQ=255)           ccGGGCAGATCCAGCACATGGTTGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTAcg                                                                            >  1:675011/1‑90 (MQ=255)            cGGGCAGATCCAGCACATGGTTGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTAcgc                                                                           <  2:957219/90‑1 (MQ=255)                cAGATCCAGCACATGGTTGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGAt                                                                       <  2:788130/90‑1 (MQ=255)                cAGATCCAGCACATGGTTGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGAt                                                                       <  2:951314/90‑1 (MQ=255)                  gATCCAGCACATGGTTGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGGGCCGGTCTTCAATTAGGGGGAtaa                                                                     >  2:453613/1‑88 (MQ=37)                   aTCCAGCACATGGTTGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGATTAc                                                                    <  1:420869/90‑1 (MQ=255)                   aTCCAGCACATGGTTGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGATTAc                                                                    >  1:9474/1‑90 (MQ=255)                      cAGCACATGGTTGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGATTACGGc                                                                 <  1:331942/90‑1 (MQ=255)                          acaTGGTTGGCGCTAACGCGTCGCAAACCCTTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGATTACGGCAttg                                                             <  1:453613/90‑1 (MQ=39)                             tGGTTGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGATTACGGCAttgttg                                                          >  1:548023/1‑90 (MQ=255)                             tGGTTGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGATTACGGCAttgttg                                                          >  1:491866/1‑90 (MQ=255)                               gTTGGCGCTAACGCGTCGCAACCCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGATTACGGCATTGTTGGc                                                        <  2:17796/90‑1 (MQ=255)                                 tGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGATTACGGCATTGTTGGCGa                                                      >  2:721883/1‑90 (MQ=255)                                  ggCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGATTACGGCATTGTTGGCGAc                                                     <  2:380868/90‑1 (MQ=255)                                    cgcTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGATTACGTCATTGTTGGCGACGc                                                   <  1:882136/90‑1 (MQ=255)                                       tAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGt                                                >  2:967741/1‑90 (MQ=255)                                              tCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATc                                         <  2:435915/90‑1 (MQ=255)                                              tCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATc                                         <  1:967741/90‑1 (MQ=255)                                                               cATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGc                        >  2:292477/1‑90 (MQ=255)                                                               cATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGc                        >  2:537339/1‑90 (MQ=255)                                                               cATCAATAAAGATAAAAATGCGCCGGTCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGc                        >  2:677221/1‑90 (MQ=255)                                                                       aaGATAAAAATGCGCCGGTCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAg                <  2:247364/90‑1 (MQ=255)                                                                            aaaaaTGCGCCGGTCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAgcgc             >  2:766456/1‑88 (MQ=255)                                                                            aaaaaTGCGCCGGTCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAgcgc             <  1:766456/88‑1 (MQ=255)                                                                            aaaaaTGCGCCGGTCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGt           <  1:169787/90‑1 (MQ=255)                                                                            aaaaaTGCGCCGGTCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGt           >  1:1005825/1‑90 (MQ=255)                                                                                aTGCGCCGGTCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGAt       <  1:639994/90‑1 (MQ=255)                                                                                   cgcCGGTCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATc                                         >  1:787726/1‑53 (MQ=255)                                                                                   cgcCGGTCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATc                                         <  2:787726/53‑1 (MQ=255)                                                                                     ccGGTCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCAct  <  2:860419/90‑1 (MQ=255)                                                                                        |                                                                                       GTGGGGATCTCCGGGCAGATCCAGCACATGGTTGGCGCTAACGCGTCGCAAACCATTTTCGCCATCAATAAAGATAAAAATGCGCCGATCTTCCAGTACGCGGATTACGGCATTGTTGGCGACGCCGTGAAGATCCTTCCGGCGCTGACCGCAGCTTTAGCGCGTTGATCCACT  >  NZ_CP009273/43962‑44135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑