Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	989,508	C→G	A261P (GCC→CCC)	ssuC ←	aliphatic sulfonate ABC transporter permease SsuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	989,508	0	C	G	100.0%	108.4 / NA	31	A261P (GCC→CCC)	ssuC	aliphatic sulfonate ABC transporter permease SsuC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (23/8);  total (23/8)

TTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGACGTCAGCCAGTTTGCCGAGCAG  >  NZ_CP009273/989449‑989592                                                            |                                                                                     ttACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAgcg                                                        <  1:323668/90‑1 (MQ=255) ttACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAgcg                                                        >  2:323668/1‑90 (MQ=255)  tACTGCATTGAGCAACAATGGCGTTCCCTGGTTCAGACGATCAGTATTCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAgcgc                                                       >  2:227450/1‑90 (MQ=255)    cTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCaa                                                     >  2:114015/1‑90 (MQ=255)         ttGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCaga                                                >  1:640241/1‑90 (MQ=255)              caacaaTGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGgcgc                                           >  2:638840/1‑90 (MQ=255)                     ggCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGgctcct                                                                                 <  2:609282/45‑1 (MQ=255)                     ggCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGgctcct                                                                                 >  1:609282/1‑45 (MQ=255)                     ggCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACa                                    >  2:653885/1‑90 (MQ=255)                        gTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCt                                 >  1:230511/1‑90 (MQ=255)                        gTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCt                                 >  2:529576/1‑90 (MQ=255)                        gTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCt                                 >  1:268763/1‑90 (MQ=255)                          gCCCTGGTTCAGACGAGCAGTATTCATACCGTGGGCTCCTTCaa                                                                             <  1:953144/44‑1 (MQ=255)                          gCCCTGGTTCAGACGAGCAGTATTCATACCGTGGGCTCCTTCaa                                                                             >  2:953144/1‑44 (MQ=255)                          gCCCTGGTTCAGACGAGCAGTATTCATACCGTGGGCTCCTTCAAATGa                                                                         <  1:829123/48‑1 (MQ=255)                          gCCCTGGTTCAGACGAGCAGTATTCATACCGTGGGCTCCTTCAAATGa                                                                         >  2:829123/1‑48 (MQ=255)                          gCCCTGGTTCAGACGAGCAGTATTCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTgc                               >  1:326068/1‑90 (MQ=255)                               ggTTCAGACGAGCAGTATTCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCt                          >  2:754229/1‑90 (MQ=255)                               ggTTCAGACGAGCAGTATTCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCt                          >  2:908356/1‑90 (MQ=255)                                   cAGACGAGCAGTATTCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGACg                      <  1:754229/90‑1 (MQ=255)                                     gACGAGCAGTATTCATACCGTTGGCTCCTTCAAATGATAAGCGGGGTTCCAGCGCAACCACAGGCCCTCTAACAGCTGCGCGCTGACGTc                    >  2:918886/1‑90 (MQ=255)                                         aGCAGTATTCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGACGTCAGcc                >  2:1042580/1‑90 (MQ=255)                                          gCAGTATTCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGACGTCAGCCa               >  2:834026/1‑90 (MQ=255)                                               aTTCATACTGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGACGTCAGCCAGTTTg          >  1:167094/1‑90 (MQ=255)                                               aTTCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGACGTCAGCCAGTTTg          >  2:310267/1‑90 (MQ=255)                                                ttCATACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGACGTCAGCCAGTTTGc         <  1:638840/90‑1 (MQ=255)                                                    tACCGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGACGTCAGCCAGTTTGCCGAg     >  2:427252/1‑90 (MQ=255)                                                      ccGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCTCTGACGTCAGCCAGTTTGCCGAGCa   <  2:268763/90‑1 (MQ=255)                                                      ccGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGACGTCAGCCAGTTTGCCGAGCa   <  1:427252/90‑1 (MQ=255)                                                       cGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGgcg                                            <  1:533821/48‑1 (MQ=255)                                                       cGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGgcg                                            >  2:533821/1‑48 (MQ=255)                                                       cGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGACGTCAGCCAGTTTGCCGAGCAg  >  2:791539/1‑90 (MQ=255)                                                       cGTGGGCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGACGTCAGCCAGTTTGCCGAGCAg  >  2:173475/1‑90 (MQ=255)                                                            |                                                                                     TTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGATAAGCCGGGTTCCAGCGCAACCAGAGGCGCTCTAACAGCTGCGCGCTGACGTCAGCCAGTTTGCCGAGCAG  >  NZ_CP009273/989449‑989592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑