Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,015,359	T→C	T51A (ACC→GCC)	ompA ←	porin OmpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,015,359	0	T	C	100.0%	97.6 / NA	28	T51A (ACC→GCC)	ompA	porin OmpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/15);  total (13/15)

TAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACCAGCCCAGTTTAGCACCAGTGTACCAGGTGTTATCTTTC  >  NZ_CP009273/1015273‑1015441                                                                                       |                                                                                   tAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGCCgg                                                                                 >  1:1040933/1‑90 (MQ=255) tAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGCCgg                                                                                 >  2:686439/1‑90 (MQ=255) tAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGCCgg                                                                                 >  1:998665/1‑90 (MQ=255)      aGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGCCGGGCCAt                                                                            >  1:674190/1‑90 (MQ=255)            aaCCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGCCGGGCCAttgttct                                                                      <  1:37497/90‑3 (MQ=255)                 aTTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGCCGGGCCATTGTTGTtgatg                                                                 <  1:267534/90‑1 (MQ=255)                      aaaGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGCCGGGCCATTGTTGTTGATGAAAcc                                                            <  1:768287/90‑1 (MQ=255)                      aaaGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGCCGGGCCATTGTTGTTGATGAAAcc                                                            <  1:613504/90‑1 (MQ=255)                                       tAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGCCGGGCCATTGTTGTTGATGAAACCGGTGTCATGGTACTggg                                           >  2:289606/1‑90 (MQ=255)                                       tAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGCCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACAggg                                           >  1:456835/1‑90 (MQ=255)                                              gTAACCACCAAAAGCACCAGCGCCCAGTTGGCTTTCATGGGCCGGACCATTGTTGTTAATGAAACCAGTGTCATGGTACTGGGACCAGcc                                    >  2:423364/1‑90 (MQ=255)                                                     ccaAAAGCACCAGCGCCCAGTTGGTTTTCATGGGCCGGACCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACCAGCCCAGTTTa                             <  2:1040933/90‑1 (MQ=255)                                                         aaGCACCAGCGCCCAGTTGGTTTTCATGGGCCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACCAGCCCAGTTTAGCAc                         <  2:619682/90‑1 (MQ=255)                                                          aGCACCAGCGCCCAGTTGGTTTTCATGGGCCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACCAGCCCAGTTTAGCAcc                        >  1:529231/1‑90 (MQ=255)                                                           gCACCAGCGCCCAGTTGGTTTTCATGGGCCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACCAGCCCAGTTTAGCACCa                       <  2:878816/90‑1 (MQ=255)                                                           gCACCAGCGCCCAGTTGGTTTTCATGGGCCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACCAGCCCAGTTTAGCACCa                       <  2:529231/90‑1 (MQ=255)                                                           gCACCAGCGCCCAGTTGGTTTTCATGGGCCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACCAGCCCAGTTTAGCACCa                       <  1:759796/90‑1 (MQ=255)                                                            cACCAGCGCCCAGTTGGTTTTCATGGGCCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACCAGCCCAGTTTAGCACCAg                      <  1:1039026/90‑1 (MQ=255)                                                               cAGCGCCCAGTTGGTTTTCATGGTCCGGGCCATTGTTGTTGATGAAACCAGCGCCATGGAACTGGGGCCAGCCCAGTTTAGCACCAgtgt                   >  2:675398/1‑90 (MQ=255)                                                                aGCGCCCAGTTGGTTTTCATGGGCCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACCAGCCCAGTTTAGc                           <  2:522184/81‑1 (MQ=255)                                                                aGCGCCCAGTTGGTTTTCATGGGCCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACCAGCCCAGTTTAGc                           >  1:522184/1‑81 (MQ=255)                                                                  cgcCCAGTTGGTTTTCATGGGCCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACCAGCCCAGTTTAGCACCAGTGTAcc                <  1:686439/90‑1 (MQ=255)                                                                   gcCCAGTTGGTTTTCATGGGCCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACCAGCCCAGTTTAGCACCAGTGTACCa               <  1:297227/90‑1 (MQ=255)                                                                     ccAGTTGGTTTTCATGGGCCGGGCCATTGTTGTTGATGAAACCAGTGTCAt                                                    >  1:825007/1‑51 (MQ=255)                                                                     ccAGTTGGTTTTCATGGGCCGGGCCATTGTTGTTGATGAAACCAGTGTCAt                                                    <  2:825007/51‑1 (MQ=255)                                                                        gTTGGTTTTCATGGGCCGGGCCATTATTGTTGATGAAACCAGTGTCATGGTACTGGGACCAGCCCa                                  <  1:359058/66‑1 (MQ=255)                                                                        gTTGGTTTTCATGGGCCGGGCCATTATTGTTGATGAAACCAGTGTCATGGTACTGGGACCAGCCCa                                  >  2:359058/1‑66 (MQ=255)                                                                                tCATGGGCCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACCAGCCCAGTTTAGCACCAGTGTACCAGGTGTTATCTTTc  >  1:560516/1‑90 (MQ=255)                                                                                       |                                                                                   TAACCAGTCGTAACCCATTTCAAAGCCAACATACGGGTTAACCTGGTAACCACCAAAAGCACCAGCGCCCAGTTGGTTTTCATGGGTCGGGCCATTGTTGTTGATGAAACCAGTGTCATGGTACTGGGACCAGCCCAGTTTAGCACCAGTGTACCAGGTGTTATCTTTC  >  NZ_CP009273/1015273‑1015441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑