Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,067,094	A→G	V43A (GTC→GCC)	rutD ←	pyrimidine utilization protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,067,094	0	A	G	100.0%	33.6 / NA	11	V43A (GTC→GCC)	rutD	pyrimidine utilization protein D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/5);  total (6/5)

TGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACC  >  NZ_CP009273/1067010‑1067161                                                                                     |                                                                    tGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGGCTAcc                                                                <  1:691503/90‑1 (MQ=255)    aGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGGATACCTGa                                                             >  2:144019/1‑90 (MQ=255)          gCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGGCTACCTGATACTcc                                                       >  1:1022708/1‑90 (MQ=255)           cTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGGCTACCTGATACTCCt                                                      <  1:509174/90‑1 (MQ=255)           cTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGGCTACCTGATACTCCt                                                      <  1:668003/90‑1 (MQ=255)                            cTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGGATACCTGATACTCCTGCTCCAGCACCGCCAGt                                     <  1:144019/90‑1 (MQ=255)                                      ctGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGGCTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAAcc                           >  2:1008246/1‑90 (MQ=255)                                             cGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGGCTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACt                    >  2:332514/1‑90 (MQ=255)                                              gtgtCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGGCTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTg                   <  1:332514/90‑1 (MQ=255)                                                            gCCGGTGCCGCGCTGGTCGTAACAGGCTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAg     >  2:440657/1‑90 (MQ=255)                                                               ggTGCCGCGCTGGTCGTAACAGGCTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGAcc  >  2:398172/1‑90 (MQ=255)                                                                                     |                                                                    TGCAGTTCCGCTGCCATCTGGGCGATACTGTAATCTTCTGCCAGCGTGTCGGGATTATTGCCGGTGCCGCGCTGGTCGTAACAGACTACCTGATACTCCTGCTCCAGCACCGCCAGTTGCGGTAACCAGTAACTGCCGCTACCCCCAAGACC  >  NZ_CP009273/1067010‑1067161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑