Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,084,094	T→C	D407G (GAT→GGT)	pgaB ←	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase PgaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,084,094	0	T	C	100.0%	92.2 / NA	26	D407G (GAT→GGT)	pgaB	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase PgaB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/9);  total (17/9)

AGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATA  >  NZ_CP009273/1084008‑1084174                                                                                       |                                                                                 agagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAAcccc                                                                               <  1:1066282/90‑1 (MQ=255) agagaCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAAcccc                                                                               <  1:564040/90‑1 (MQ=255)        gTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAACCCCAGCTTaa                                                                        >  2:881399/1‑90 (MQ=255)        gTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAACCCCAGCTTaa                                                                        >  1:677857/1‑90 (MQ=255)            tatTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAACCCCAGCTTAATAcc                                                                    >  1:841280/1‑90 (MQ=255)                    aGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCa                                                            >  2:75257/1‑90 (MQ=255)                    aGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCa                                                            >  1:53839/1‑90 (MQ=255)                          aaTTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATa                                                      >  2:288976/1‑90 (MQ=255)                            tttGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGa                                                    <  2:78552/90‑1 (MQ=255)                            tttGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGa                                                    <  1:75257/90‑1 (MQ=255)                              tgtgCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGATg                                                  <  2:545470/90‑1 (MQ=255)                                   ttttttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGATGTTTac                                             >  2:435578/1‑90 (MQ=255)                                    tttttCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGATGTTTaca                                            >  1:610944/1‑90 (MQ=255)                                           ccccTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGc                                     <  1:292162/90‑1 (MQ=255)                                                   ggTAAGTATTTTACTCGCGTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGt                             >  1:145927/1‑90 (MQ=255)                                                     tAAGTATTTTACTCGCGTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTaa                           >  1:923097/1‑90 (MQ=255)                                                          aTTTTACTCGCGTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGa                                                    >  2:268781/1‑60 (MQ=255)                                                          aTTTTACTCGCGTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGa                                                    <  1:268781/60‑1 (MQ=255)                                                          aTTTTACTCGCGTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGcc                      >  2:576439/1‑90 (MQ=255)                                                                  cgcgTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGa                                                    >  2:40216/1‑52 (MQ=255)                                                                  cgcgTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGa                                                    <  1:40216/52‑1 (MQ=255)                                                                  cgcgTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAAcc              >  1:106411/1‑90 (MQ=255)                                                                   gcgTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAAccc             <  1:435578/90‑1 (MQ=255)                                                                    cgTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCg                                    <  1:716756/66‑1 (MQ=255)                                                                    cgTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCg                                    >  2:716756/1‑66 (MQ=255)                                                                    cgTTAATGTGGGATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCg            <  2:773407/90‑1 (MQ=255)                                                                             gggATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAat   >  1:178211/1‑90 (MQ=255)                                                                              ggATCTAAACCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAata  >  2:47616/1‑90 (MQ=255)                                                                                       |                                                                                 AGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCACGCATAGATGTTTACACCTGAGCGGGTACGTAATTGCCAGGCAACCCGACTAAAAATA  >  NZ_CP009273/1084008‑1084174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑