| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,343,839 | T→C | T176A (ACA→GCA) | yciW ← | CMD domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,343,839 | 0 | T | C | 95.5% | 68.9 / ‑4.6 | 22 | T176A (ACA→GCA) | yciW | CMD domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (8/13); total (9/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGT > NZ_CP009273/1343755‑1343910 | gTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCcgcg > 1:784949/1‑90 (MQ=255) ggCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCGCGCCTGAAAGcc > 1:464509/1‑90 (MQ=255) tAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCGCGCCTGAAAGCCAATa < 1:511717/90‑1 (MQ=255) tAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCGCGCCTGAAAGCCAATa < 2:1054902/90‑1 (MQ=255) ctcCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCGCGCCTGAAAGCCAATAAAACCAATGAt < 2:464509/90‑1 (MQ=255) tcCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCGCGCCTGAAAGCCAATAAAACCAATGAtt > 1:194064/1‑90 (MQ=255) ccAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATcac < 2:272634/90‑1 (MQ=255) aGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATcacac < 1:871209/90‑1 (MQ=255) aGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATcacac < 2:386554/90‑1 (MQ=255) aGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATcacac < 1:267507/90‑1 (MQ=255) aGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATcacac < 1:267506/90‑1 (MQ=255) gCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTa > 1:590389/1‑79 (MQ=255) gCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTa < 2:590389/79‑1 (MQ=255) gTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGt > 1:197431/1‑90 (MQ=255) ggATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCTCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAg > 1:152430/1‑90 (MQ=255) ggATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCGCGCCTGaaa < 1:753743/47‑1 (MQ=255) ggATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCGCGCCTGaaa < 1:565906/47‑1 (MQ=255) ggATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCGCGCCTGaaa > 2:565906/1‑47 (MQ=255) ggATGCCCGAGATAAGCCTGAAATGTCGCAATTGCCCGCGCCTGaaa > 2:753743/1‑47 (MQ=255) aTAAGCCTGAAATGTCGCAATTGCCCTCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCCCACAGTCCCAGGCAGTAAGAcc < 2:152430/90‑1 (MQ=255) gTCGCAATTGCCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGt > 1:935830/1‑82 (MQ=255) gTCGCAATTGCCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGt < 2:935830/82‑1 (MQ=255) | GTGACGCGTCGGCGTAGTTTTGTATCTCCAGCCCGGGTAACCAGCGTACCGGATGCCCGAGATAAGCCTGAAATGTCGCAATTGTCCGCGCCTGAAAGCCAATAAAACCAATGATTTGGCTAATGATCACACAGTCCCAGGCAGTAAGACCGACGT > NZ_CP009273/1343755‑1343910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |