| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 100,843 | T→C | H125H (CAT→CAC) | ftsA → | cell division protein FtsA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 100,843 | 0 | T | C | 97.0% | 106.9 / ‑4.0 | 33 | H125H (CAT→CAC) | ftsA | cell division protein FtsA |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (12/20); total (12/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
TGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGC > NZ_CP009273/100758‑100925 | tGCCTATTTCTGAAGAAGAAGAGACGCAAGAAGATGTGGAAAACGTCTTCCATACCGCGAAAGCGGTGCGTGTGCGCGATGAGCACCgtg > 2:732005/1‑90 (MQ=255) gCCTATTTCTGAAGAAGAAGGGACGCAAGAAGATGGGGAAAACGTCTTCCATACCGCGAAATCGGTGCGGGTGCGCGATGAGCACCgtgt > 2:292569/1‑90 (MQ=255) cTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGtgc > 1:227726/1‑90 (MQ=255) cTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGtgc > 1:972693/1‑90 (MQ=255) cTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCg < 2:962698/79‑1 (MQ=255) cTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCg > 1:962698/1‑79 (MQ=255) agaagaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGAt < 2:206283/90‑1 (MQ=255) agaagaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGAt < 2:200690/90‑1 (MQ=255) agaagaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGAt < 1:378096/90‑1 (MQ=255) agaagTGACGCAAGAAGATGTGGAAAACTTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGATccc > 1:661925/1‑90 (MQ=255) agaagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGATccc > 1:73109/1‑90 (MQ=255) aagTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGATCCCGc < 2:251543/90‑1 (MQ=255) tGACGCAAGAAGATGTGGAAAACTTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGATCCCGCAag < 2:661925/90‑1 (MQ=255) tGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGATCCCGCAag < 2:41655/90‑1 (MQ=255) gaTGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGAtt < 2:1051639/90‑1 (MQ=255) aTGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTg < 1:1006788/90‑1 (MQ=255) aTGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTg < 2:972693/90‑1 (MQ=255) tgtgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGAATGTGATCCCGCAAGAGTATGCGATTg < 2:437546/89‑1 (MQ=255) tgtgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGAATGTGATCCCGCAAGAGTATGCGATTg > 1:437546/1‑89 (MQ=255) gtgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGAc < 1:517861/90‑1 (MQ=255) tgGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTTCATGTGATCCCGCAAGAGTATGCGATTGACt > 1:794696/1‑90 (MQ=255) cgtcgtCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGa < 1:925520/90‑1 (MQ=255) tcgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAg < 2:404494/90‑1 (MQ=255) cgtcCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAgg < 2:261188/90‑1 (MQ=255) gtcCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGtgc > 1:599741/1‑46 (MQ=255) gtcCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGtgc < 2:599741/46‑1 (MQ=255) cATACCGCGAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATc < 2:306302/90‑1 (MQ=255) gcgAAATCGGTTCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAAt > 2:666703/1‑90 (MQ=255) gcgAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAAt < 2:146365/90‑1 (MQ=255) cgAAATCGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATc > 1:251502/1‑90 (MQ=255) cGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAg < 1:392347/90‑1 (MQ=255) cGGTGCGTGTGCGCGATGAGCACCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAg < 1:313514/90‑1 (MQ=255) aTGAGCACCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGc < 2:973108/90‑1 (MQ=255) | TGCCTATTTCTGAAGAAGAAGTGACGCAAGAAGATGTGGAAAACGTCGTCCATACCGCGAAATCGGTGCGTGTGCGCGATGAGCATCGTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGC > NZ_CP009273/100758‑100925 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |