Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,393,322	A→T	D154E (GAT→GAA)	fnr ←	fumarate/nitrate reduction transcriptional regulator Fnr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,393,322	0	A	T	100.0%	25.5 / NA	11	D154E (GAT→GAA)	fnr	fumarate/nitrate reduction transcriptional regulator Fnr
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (5/6);  total (5/6)

ACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTT  >  NZ_CP009273/1393247‑1393404                                                                            |                                                                                   acgGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGTTCGCCTTTGATTTc                                                                      <  1:366984/90‑1 (MQ=255)      aCAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGTTCGCCTTTGATTTCACCGc                                                                 <  1:237378/90‑1 (MQ=255)      aCAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGTTCGCCTTTGATTTCACCGc                                                                 <  1:431071/90‑1 (MQ=255)                   gAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGTTc                                                                                  <  1:736669/60‑1 (MQ=255)                   gAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGTTc                                                                                  >  2:736669/1‑60 (MQ=255)                   gAATGCAGACAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGTTCGCCTTTGATTTCACCGCTCATCAg                                                          <  1:631323/84‑1 (MQ=255)                   gAATGCAGACAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGTTCGCCTTTGATTTCACCGCTCATCAg                                                          >  2:631323/1‑84 (MQ=255)                                           aTTTTTCTTCGACAACAGCAGGATCATGTCCTGTTCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCAt                            <  2:482104/90‑1 (MQ=255)                                            tttttCTTCGACAACAGCAGGATCATGTCCTGTTCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTTCTGACGCAGATTCGGCAtt                           >  1:966528/1‑90 (MQ=255)                                                                    aTGACCTGTTCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGtt   >  2:971512/1‑90 (MQ=255)                                                                     tGTCCTGTTCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGttt  >  2:565649/1‑90 (MQ=255)                                                                            |                                                                                   ACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTT  >  NZ_CP009273/1393247‑1393404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑