Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,494,141	A→G	F189S (TTT→TCT)	ydcK ←	YdcK family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,494,141	0	A	G	100.0%	122.1 / NA	34	F189S (TTT→TCT)	ydcK	YdcK family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/16);  total (18/16)

CTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGACAATACGCGAG  >  NZ_CP009273/1494055‑1494221                                                                                       |                                                                                 cTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCagaaa                                                                               >  2:975341/1‑90 (MQ=255)            gcgTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGa                                                                    <  2:779294/90‑1 (MQ=255)                gACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGtt                                                                >  1:190304/1‑90 (MQ=255)                gACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGtt                                                                <  2:794594/90‑1 (MQ=255)                    gTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAAt                                                            <  1:590292/90‑1 (MQ=255)                    gTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAAt                                                            <  2:190304/90‑1 (MQ=255)                    gTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAAt                                                            <  1:707717/90‑1 (MQ=255)                           ttAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCa                                                     >  1:40394/1‑90 (MQ=255)                                            ccAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATc                                    <  2:633973/90‑1 (MQ=255)                                            ccAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATc                                    <  2:1039520/90‑1 (MQ=255)                                               gACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGa                                                                    >  2:742967/1‑55 (MQ=255)                                               gACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGa                                                                    <  1:742967/55‑1 (MQ=255)                                               gACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGacgacg                                         <  1:129260/82‑1 (MQ=255)                                               gACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGacgacg                                         >  2:129260/1‑82 (MQ=255)                                                     gTTCTCTTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAAt                           >  2:33295/1‑90 (MQ=255)                                                     gTTCTCTTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAAt                           >  2:299715/1‑90 (MQ=255)                                                         tctTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTtgt                       >  2:154239/1‑90 (MQ=255)                                                          ctTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTtgtg                      >  1:487906/1‑90 (MQ=255)                                                          ctTCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTtgtg                      >  2:164985/1‑90 (MQ=255)                                                            tCATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGcc                    <  1:33295/90‑1 (MQ=255)                                                             cATTACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCt                   >  1:635570/1‑90 (MQ=255)                                                                tACCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtggt                <  2:915689/90‑1 (MQ=255)                                                                 aCCTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtggtg               <  1:81331/90‑1 (MQ=255)                                                                  ccTTCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtggtgg              <  2:487906/90‑1 (MQ=255)                                                                    ttCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAAt                                                            <  2:741/42‑1 (MQ=255)                                                                    ttCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAAt                                                            >  1:741/1‑42 (MQ=255)                                                                    ttCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCAt                               <  2:482130/71‑1 (MQ=255)                                                                    ttCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCAt                               >  1:482130/1‑71 (MQ=255)                                                                    ttCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGAc            >  1:679865/1‑90 (MQ=255)                                                                    ttCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGAc            >  2:880122/1‑90 (MQ=255)                                                                    ttCAATACTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGAc            >  1:792492/1‑90 (MQ=255)                                                                           cTGGCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGACAATAcgc     >  1:22243/1‑90 (MQ=255)                                                                             ggCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGACAATACGCGa   >  2:177374/1‑90 (MQ=255)                                                                             ggCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGACAATACGCGa   >  2:129366/1‑90 (MQ=255)                                                                              gCAAAATCAGAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGACAATACGCGAg  <  1:394999/90‑1 (MQ=255)                                                                                       |                                                                                 CTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTGGACAATACGCGAG  >  NZ_CP009273/1494055‑1494221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑