| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,759,822 | A→G | F23S (TTC→TCC) | menI ← | 1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,759,822 | 0 | A | G | 96.9% | 110.0 / NA | 32 | F23S (TTC→TCC) | menI | 1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (12/19); minor base C (0/1); total (12/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATG‑TTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTA > NZ_CP009273/1759735‑1759905 | aaCCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGGAc > 1:205360/1‑90 (MQ=255)aaCCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGGAc < 2:205360/90‑1 (MQ=255) ctgctTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGGACCCCACCATG‑tt < 2:130372/90‑1 (MQ=255) ctactTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGGACCCCACCATG‑tt < 1:743444/87‑1 (MQ=255) gctTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGGAc < 2:98659/77‑1 (MQ=255) gctTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGGAc > 1:98659/1‑77 (MQ=255) gctTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGGACCCCACCATG‑TTTc < 2:619551/90‑1 (MQ=255) tGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGGACCCCACCATG‑TTTCCTTc < 1:416032/90‑1 (MQ=255) gTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGGACCCCACCATG‑TTTCCTTCa < 2:676428/90‑1 (MQ=255) cgAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGGACCCCACCATG‑TTTCCTTCACCCAt < 1:1052576/90‑1 (MQ=255) cgAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGGACCCCACCATG‑TTTCCTTCACCCAt > 1:837782/1‑90 (MQ=255) gAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGGACCCCACCATG‑TTTCCTTCACCCATa < 1:87105/90‑1 (MQ=255) tGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGGACCCCACCATG‑TTTCCTTCACCCATAGCATTCAGTGCTTc > 2:966065/1‑90 (MQ=255) tgtCATCACCAATATGTTCAAAGCGAATATCCAGGGACCCCACCATG‑TTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTcc < 1:701473/90‑1 (MQ=255) gtCATCACCAATATGTTCAAAGCGAATATCCAGGGACCCCACCATGTTTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTcc > 1:620271/1‑90 (MQ=255) ccAATATGTTCAAAGCGAATATCCAGGGACCCCACCATG‑TTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCat > 1:20906/1‑90 (MQ=255) ccAATATGTTCAAAGCGAATATCCAGGGACCCCACCATG‑TTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCat < 1:6663/90‑1 (MQ=255) ccAATATGTTCAAAGCGAATATCCAGGCCCCCCCCCATG‑TTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCat < 1:232497/90‑1 (MQ=255) atatGTTCAAAGCGAATATCCAGGGACCCCACCATGTTTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCatat < 2:620271/90‑1 (MQ=255) atatGTTCAAAGCGAATATCCAGGGACCCCACCATG‑TTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATc > 2:95051/1‑90 (MQ=255) ttCAAAGCGAATATCCAGGGACCCCACCATG‑TTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTta > 2:634520/1‑90 (MQ=255) cAAAGCGAATATCCAGGGACCCCACCATG‑TTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTtaat > 1:306963/1‑90 (MQ=255) aaGCGAATATCCAGGGACCCCACCATG‑TTTCCTTCACCCa < 2:413638/40‑1 (MQ=255) aaGCGAATATCCAGGGACCCCACCATG‑TTTCCTTCACCCa > 1:413638/1‑40 (MQ=255) aaGCGAATATCCAGGGACCCCACCATG‑TTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTtaataa < 2:901797/90‑1 (MQ=255) gCGAATATCCAGGGACCCCACCATG‑TTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAAtc < 2:20906/90‑1 (MQ=255) gCGAATATCCAGGGACCCCACCATG‑TTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAAtc < 1:634520/90‑1 (MQ=255) aaTATCCAGGGACCCCACCATG‑TTTCCTTCACCCATAGCATTCAGt < 2:504184/46‑1 (MQ=255) aaTATCCAGGGACCCCACCATG‑TTTCCTTCACCCATAGCATTCAGt > 1:504184/1‑46 (MQ=255) aaTATCCAGGGACCCCACCATG‑TTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTcc > 2:340419/1‑90 (MQ=255) tCCAGGGACCCCACCATG‑TTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTa < 1:704213/90‑1 (MQ=255) tCCAGGGACCCCACCATG‑TTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTa < 1:1025224/90‑1 (MQ=255) | AACCCGAAAGGCTGCTTTGTCCGCGAGTCTACTGGCATTGTCGCTTCAAGGGTGTCATCACCAATATGTTCAAAGCGAATATCCAGGAACCCCACCATG‑TTTCCTTCACCCATAGCATTCAGTGCTTCCAGGGTGATTTTCCGTTTCCATATCATTTAATAATCTCCAGTA > NZ_CP009273/1759735‑1759905 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |