Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,907,320	A→G	F585S (TTC→TCC)	prc ←	carboxy terminal‑processing peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,907,320	0	A	G	100.0%	92.2 / NA	27	F585S (TTC→TCC)	prc	carboxy terminal‑processing peptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/11);  total (16/11)

TCTCACGCACAGCGTAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAATCTCCTGATTTCA  >  NZ_CP009273/1907234‑1907398                                                                                       |                                                                               tctcACGCACAGCGTAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGGACt                                                                             >  1:266308/1‑90 (MQ=255)   tcACGCACAGCGTAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGGACTCa                                                                           >  1:457553/1‑90 (MQ=255)        cacaGCGTAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGGACTCAGGATc                                                                      >  2:709138/1‑90 (MQ=255)          caGCGTAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGGACTCAGGATCtt                                                                    >  1:809048/1‑90 (MQ=255)            gCGTAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGGACTCAGGATCTTTc                                                                  >  1:1054552/1‑90 (MQ=255)            gCGTAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGGACTCAGGATCTTTc                                                                  >  2:851784/1‑90 (MQ=255)            gCGTAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGGACTCAGGATCTTTc                                                                  >  2:339743/1‑90 (MQ=255)                    cAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGGACTCAGGATCTTTCGCGATAcg                                                          <  1:325529/90‑1 (MQ=255)                    cAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGGACTCAGGATCTTTCGCGATAcg                                                          >  2:80143/1‑90 (MQ=255)                      gagaAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGGACTCAGGATCTTTCGCGATAcgcg                                                        <  2:578350/90‑1 (MQ=255)                      gagaAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGGACTCAGGATCTTTCGCGATAcgcg                                                        >  2:773611/1‑90 (MQ=255)                      gagaAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGACATCCTTCATGATGTTCTGGGACTCAGGATCTTTCGCGATAcgcg                                                        >  1:358164/1‑90 (MQ=255)                       agaAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGGACTCAGGATCTTTCGCGATACgcgc                                                       <  2:809048/90‑1 (MQ=255)                         aaaCGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGGACTCAGGATCTTTCGCGATACGCGCAt                                                     <  1:709138/90‑1 (MQ=255)                                        tGTCCTTCATAGCGTTGAAGCTCTCGATATCCTTCATGATGTTCTGGGACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTcagca                                      >  1:848529/1‑90 (MQ=255)                                             ttCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGGACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTcc                                 <  2:790456/90‑1 (MQ=255)                                             ttCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGGACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTcc                                 <  2:787469/90‑1 (MQ=255)                                                     gTTGAAGCGCGCGATATCCTTCATGATGTTCTGGGACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCaaa                         <  1:339743/90‑1 (MQ=255)                                                     gTTGAAGCGCGCGATATCCTTCATGATGTTCTGGGACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCaaa                         <  1:851784/90‑1 (MQ=255)                                                             gcgcgATATCCTTCATGATGTTCTGGGACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTa                 >  1:103305/1‑90 (MQ=255)                                                             gcgcgATATCCTTCATGATGTTCTGGGACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTa                 >  2:804543/1‑90 (MQ=255)                                                                    atCCTTCATGATGTTCTGGGACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAATCTcc          <  1:986942/90‑1 (MQ=255)                                                                        ttCATGATGTTCTGGGACTCAGGATCTTTCGCGATAcg                                                          >  1:159637/1‑38 (MQ=38)                                                                        ttCATGATGTTCTGGGACTCAGGATCTTTCGCGATAcg                                                          <  2:159637/38‑1 (MQ=38)                                                                            tgatgTTCTGGGACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAATCTCCTGATTTca  >  1:290929/1‑90 (MQ=255)                                                                               tgTTCTGGGACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAATCTCCTGAtt     <  1:276145/84‑1 (MQ=255)                                                                               tgTTCTGGGACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAATCTCCTGAtt     >  2:276145/1‑84 (MQ=255)                                                                                       |                                                                               TCTCACGCACAGCGTAATTCAGAGAAACGATATTGCGCTTGTCCTTCATAGCGTTGAAGCGCGCGATATCCTTCATGATGTTCTGGAACTCAGGATCTTTCGCGATACGCGCATTATGTTCCTTCAGCAGCTCCGGTTCAAAGGCCGTTAAATCTCCTGATTTCA  >  NZ_CP009273/1907234‑1907398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑