Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	136,175	T→C	E513G (GAA→GGA)	gcd ←	quinoprotein glucose dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	136,175	0	T	C	100.0%	75.9 / NA	22	E513G (GAA→GGA)	gcd	quinoprotein glucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/9);  total (13/9)

TCTTTCGTCGGACGGAAGCTCAGTTCAGAAAACGGTTGAGTTGGGGTTACGTAATCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTTCCGGTGCCGGAACCACCAGTTCGCCATTACGACGATCGAGCACAAAAATGTTGCCGGTTTTCGCCGGAGCGTAAATAACTGGCACT  >  NZ_CP009273/136088‑136261                                                                                        |                                                                                       tCTTTCGTCGGACGGAAGCTCAGTTCAGAAAACGGTTGAGTTGGGGTTACGTAATCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTccc                                                                                      <  1:975858/90‑1 (MQ=255) tCTTTCGTCGGACGGAAGCTCAGTTCAGAAAACGGTTGAGTTGGGGTTACGTAATCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTccc                                                                                      <  1:620983/90‑1 (MQ=255)    ttCGTCGGACGGAAGCTCAGTTCAGAAAACGGTTGAGTTGGGGTTACGTAATCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTCCCGGt                                                                                   >  2:594925/1‑90 (MQ=255)      cgtcgGACGGAAGCTCAGTTCAGAAAACGGTTGAGTTGGGGTTACGTAATCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTCCCGGTGc                                                                                 <  1:20047/90‑1 (MQ=255)                     cAGTTCAGAAAACGGTTGAGTTGGGGTTACGTAATCGCCTTTCGCTTCACCTTGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTc                                                                  >  1:7322/1‑90 (MQ=255)                       gTTCAGAAAACGGTTGAGTTGGGGTTACGTAATCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTCGc                                                                >  1:502372/1‑90 (MQ=255)                       gTTCAGAAAACGGTTGAGTTGGGGTTACGTAATCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTCGc                                                                <  1:594925/90‑1 (MQ=255)                               aaCGGTTGAGTTGGGGTTACGTAATCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTc                                                                  <  2:795506/80‑1 (MQ=255)                               aaCGGTTGAGTTGGGGTTACGTAATCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTc                                                                  >  1:795506/1‑80 (MQ=255)                                                tACGTAATCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTCGCCATTACGACGATCGAGCACAAAAAt                                       >  2:990291/1‑90 (MQ=255)                                                     aaTCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTCGCCATTACGACGATCGAGCACAAAAATGTTGc                                  >  2:640254/1‑90 (MQ=255)                                                      aTCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTCGCCATTACGACGATCg                                                  <  1:442673/73‑1 (MQ=255)                                                      aTCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTCGCCATTACGACGATCg                                                  >  2:442673/1‑73 (MQ=255)                                                      aTCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTCGCCATTACGACGATCGAGc                                               <  2:265905/76‑1 (MQ=255)                                                      aTCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTCGCCATTACGACGATCGAGc                                               >  1:265905/1‑76 (MQ=255)                                                      aTCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTCGCCATTACGACGATCGAGCACAAAAATGTTGcc                                 >  1:103245/1‑90 (MQ=255)                                                      aTCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTCGCCATTACGACGATCGAGCACAAAAATGTTGcc                                 >  2:673385/1‑90 (MQ=255)                                                                 cTGCACCTTGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTCGCCATTACGACGATCGAGCACAAAAATGTTGCCGGTTTTCGCCg                      <  2:109150/90‑1 (MQ=255)                                                                 cTGCACCTTGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTCGCCATTACGACGATCGAGCACAAAAATGTTGCCGGTTTTCGCCg                      <  2:551931/90‑1 (MQ=255)                                                                        ttGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTc                                                                  >  1:452092/1‑39 (MQ=255)                                                                        ttGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTCGCCATTACGACGATCGAGCACAAAAATGTTGCCGGTTTTCGCCGGAGCGTa               >  2:768605/1‑90 (MQ=255)                                                                        ttGGGGAACCGGTTTTCCCGGTGCCGGAACCACCAGTTCGCCATTACGACGATCGAGCACAAAAATGTTGCCGGTTTTCGCCGGAGCGTa               >  1:379030/1‑90 (MQ=255)                                                                          ggggAACCGGTTTTCCCGGTGCCGGAACCACCAGTTCGCCATTACGACGATCGAGCACAAAAATGTTGCCGGTTTTCGCCGGAGCGTaaa             <  2:265665/90‑1 (MQ=255)                                                                          ggggAACCGGTTTTCCCGGTGCCGGAACCACCAGTTCGCCATTACGACGATCGAGCACAAAAATGTTGCCGGTTTTCGCCGGAGCGTaaa             <  1:336182/90‑1 (MQ=255)                                                                                     tttcCCGGTGCCGGAACCACCAGTTCGCCATTACGACGATCGAGCACAAAAATGTTGCCGGTTTTCGCCGGAGCGTAAATAACTGGCACt  <  2:920033/90‑1 (MQ=255)                                                                                        |                                                                                       TCTTTCGTCGGACGGAAGCTCAGTTCAGAAAACGGTTGAGTTGGGGTTACGTAATCGCCTTTCGCTGCACCTTGGGGAACCGGTTTTTCCGGTGCCGGAACCACCAGTTCGCCATTACGACGATCGAGCACAAAAATGTTGCCGGTTTTCGCCGGAGCGTAAATAACTGGCACT  >  NZ_CP009273/136088‑136261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑