Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,943,712	T→G	E356D (GAA→GAC)	aspS ←	aspartate‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,943,712	0	T	G	100.0%	98.0 / NA	28	E356D (GAA→GAC)	aspS	aspartate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/13);  total (15/13)

GGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTC  >  NZ_CP009273/1943625‑1943793                                                                                        |                                                                                  ggCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGcggtc                                                                                 <  2:354118/90‑1 (MQ=255)  gCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGcggtcg                                                                                >  2:721790/1‑90 (MQ=255)  gCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGcggtcg                                                                                >  1:334310/1‑90 (MQ=255)  gCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGcggtcg                                                                                >  1:35837/1‑90 (MQ=255)  gCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGcggtcg                                                                                >  2:44383/1‑90 (MQ=255)          aTCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGGTCGTTAACttt                                                                        <  1:660755/90‑1 (MQ=255)            ccAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGa                                                                      >  1:145104/1‑90 (MQ=255)               ggATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGt                                                                   <  2:708600/90‑1 (MQ=255)               ggATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGt                                                                   >  2:465119/1‑90 (MQ=255)                    tCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGcc                                                              >  2:694609/1‑90 (MQ=255)                       tCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGa                                                           <  1:92456/90‑1 (MQ=255)                         gatgatTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGAcc                                                         >  2:80877/1‑90 (MQ=255)                              tttCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTcg                                                    <  2:145104/90‑1 (MQ=255)                                 cTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTCgcgc                                                 >  1:717482/1‑90 (MQ=255)                                             aCTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTaa                                     >  1:759720/1‑90 (MQ=255)                                              ctccGCTACCGGGCTGTTGATGCCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGGCCTTTCGCGCCGTAGATTTTAAc                                    <  1:905243/87‑1 (MQ=255)                                              cTTCGATACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAAc                                    <  2:451171/90‑1 (MQ=255)                                                  gCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGacg                                >  2:145501/1‑88 (MQ=255)                                                  gCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAg                                <  2:778833/90‑1 (MQ=255)                                                             gTTGATACCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATtc                     <  1:465119/90‑1 (MQ=255)                                                               tGATACCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATtcgt                   >  2:554308/1‑90 (MQ=255)                                                                  tACCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTcgcg                                                  <  2:631965/56‑1 (MQ=255)                                                                  tACCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTcgcg                                                  >  1:631965/1‑56 (MQ=255)                                                                   aCCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGAt               >  1:115015/1‑90 (MQ=255)                                                                   aCCTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGAt               >  2:477360/1‑90 (MQ=255)                                                                    ccTTCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATc              <  1:80877/90‑1 (MQ=255)                                                                       tCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGc           >  2:604426/1‑90 (MQ=255)                                                                       tCCAGACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTCGAGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGc           >  1:108115/1‑90 (MQ=255)                                                                           gACCTTTCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTAc       <  2:35837/90‑1 (MQ=255)                                                                               tttCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGt   >  1:149167/1‑90 (MQ=255)                                                                                ttCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACg                                   <  2:163748/57‑1 (MQ=255)                                                                                ttCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACg                                   >  1:163748/1‑57 (MQ=255)                                                                                ttCGCGCGGTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTc  <  1:554308/90‑1 (MQ=255)                                                                                        |                                                                                  GGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATACCTTCCAGACCTTTCGCGCGTTCGTTAACTTTGATGTAAGCCAGACCTTTCGCGCCGTAGATTTTAACGAAGTTACCGTATTCGTCGATCTGCTTACGGGTC  >  NZ_CP009273/1943625‑1943793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑