Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,090,333	A→G	D80G (GAC→GGC)	hisIE →	bifunctional phosphoribosyl‑AMP cyclohydrolase/phosphoribosyl‑ATP diphosphatase HisIE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,090,333	0	A	G	100.0%	67.7 / NA	22	D80G (GAC→GGC)	hisIE	bifunctional phosphoribosyl‑AMP cyclohydrolase/phosphoribosyl‑ATP diphosphatase HisIE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/8);  total (14/8)

CGCGCACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGTTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACC  >  NZ_CP009273/2090258‑2090418                                                                            |                                                                                      cgcgCACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGGCTGCGACAACGaca                                                                         <  2:937700/90‑1 (MQ=255)  gcgcACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGCGTATTGCCCCGGGCTGCGACAACGacac                                                                        >  2:311604/1‑90 (MQ=255)  gcgcACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGGCTGCGACAACGacac                                                                        >  1:563693/1‑90 (MQ=255)               cGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGGCTGCGACAACGACACGTTACTGGTGCTg                                                           >  1:712880/1‑90 (MQ=255)               cGACTGGGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGGCTGCGACAACGACACGTTACTGGTGCTg                                                           >  1:1040202/1‑90 (MQ=255)                 aCTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGGCTGCGACAACGACACGTTACTGGTGCTGGc                                                         >  2:803475/1‑90 (MQ=255)                 aCTGTGGACCAAAGGCGAAACGGCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGGCTGCGACAACGACACGTTACTGGTGCTGGc                                                         >  2:464243/1‑90 (MQ=255)                   tgtgGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGGCTGCGACAACGACACGTTACTGGTGCTGGCGa                                                       >  2:267543/1‑90 (MQ=255)                    gtgGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGGCTGCGACAACGACACGTTACTGGTGCTGGCGaa                                                      >  1:566924/1‑90 (MQ=255)                           aaaGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGGCTGCGACAACGACACGTTACTGGTGCTGGCGAATCCCATc                                               >  2:259260/1‑90 (MQ=255)                                cGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGGCTGCGACAACGACACGTTACTGGTGCTGGCGAATCCCATCGGccc                                          <  1:259260/90‑1 (MQ=255)                                              aTTTCCTCAACGTAGTGAGTATTGCCCCGGGCTGCGACAACGACACGTTACTGGTGCTGGCAAACCCCATCGGCCCGCCCTGCCACAAAg                            <  2:1040202/90‑1 (MQ=255)                                                    tCAACGTAGTGAGTATTGCCCCGGGCTGCGACAACGACACGTTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACca                      <  2:712880/90‑1 (MQ=255)                                                          tAGTGAGTATTGCCCCGGGCTGCGACAACGACACGTTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGtt                >  2:965590/1‑90 (MQ=255)                                                             tGAGTATTGCCCCGGGCTGCGACAACGACACGTTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCt             <  1:192488/90‑1 (MQ=255)                                                                 tATTGCCCCGGGCTGCGACAACGACACGTTACTGGTGCTGGAGAATCCCATCGGccc                                          <  2:332775/57‑1 (MQ=255)                                                                 tATTGCCCCGGGCTGCGACAACGACACGTTACTGGTGCTGGAGAATCCCATCGGccc                                          >  1:332775/1‑57 (MQ=255)                                                                     gCCCCGGGCTGCGACAACGACACGTTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGac     >  2:101041/1‑90 (MQ=255)                                                                      ccccGGGCTGCGACAACGCCACGTTACGGGTGCGGGCGAACCCCATCGCCCCGCCCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGaca    <  2:366034/90‑1 (MQ=255)                                                                        ccGGGCTGCGACAACGACACGTTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGac     >  2:690286/1‑87 (MQ=255)                                                                        ccGGGCTGCGACAACGACACGTTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGac     <  1:690286/87‑1 (MQ=255)                                                                        ccGGGCTGCGACAACGACACGTTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACAcc  >  1:366034/1‑90 (MQ=255)                                                                            |                                                                                      CGCGCACTAAACAGCGACTGTGGACCAAAGGCGAAACGTCGGGCAATTTCCTCAACGTAGTGAGTATTGCCCCGGACTGCGACAACGACACGTTACTGGTGCTGGCGAATCCCATCGGCCCGACCTGCCACAAAGGCACCAGCAGTTGCTTCGGCGACACC  >  NZ_CP009273/2090258‑2090418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑