Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,449,226	A→G	intergenic (‑101/+580)	yfcV ← / ← sixA	fimbrial protein/phosphohistidine phosphatase SixA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,449,226	0	A	G	100.0%	222.8 / NA	61	intergenic (‑101/+580)	yfcV/sixA	fimbrial protein/phosphohistidine phosphatase SixA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/23);  total (38/23)

ATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTT  >  NZ_CP009273/2449142‑2449311                                                                                     |                                                                                       ataaataaTTAAAATATAAACATTGCAATACATTGATTCAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑aa                                                                                  >  1:874556/1‑90 (MQ=255)                       ttGCAATACATTGATTCAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTGGTAAAAAATga                                                            >  1:420746/1‑89 (MQ=255)                       ttGCAATACATTGATTCAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGt                                                            >  2:155629/1‑90 (MQ=255)                        tGCAATACATTGATTCAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTa                                                           >  1:203366/1‑90 (MQ=255)                              aCATTGATTCAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGtt                                                     <  2:203366/90‑1 (MQ=255)                                 ttGATTCAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGgt                                                  >  1:22415/1‑90 (MQ=255)                                 ttGATTCAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGgt                                                  >  1:259759/1‑90 (MQ=255)                                   gATTCAGTTAATAGCCAATGTTTATACCCAGCCATTGAATACGGCAGGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGgtgt                                                >  2:187964/1‑90 (MQ=255)                                   gATTCAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGgtgt                                                <  1:572355/90‑1 (MQ=255)                                   gATTCAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGgtgt                                                >  2:15601/1‑90 (MQ=255)                                        aGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGgtgt                                                <  2:134489/85‑1 (MQ=255)                                        aGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGgtgt                                                >  1:134489/1‑85 (MQ=255)                                        aGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTgg                                              <  1:634989/87‑1 (MQ=255)                                        aGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGt                                           >  1:293008/1‑90 (MQ=255)                                        aGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGt                                           >  2:744285/1‑90 (MQ=255)                                        aGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGt                                           <  1:15601/90‑1 (MQ=255)                                        aGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGt                                           >  1:103950/1‑90 (MQ=255)                                        aGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGGGTgg                                              >  2:634989/1‑87 (MQ=255)                                         gTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTc                                          >  1:710802/1‑90 (MQ=255)                                             aTAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAAt                                      <  2:714197/90‑1 (MQ=255)                                               aGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAg                                    <  1:104499/90‑1 (MQ=255)                                                  cAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCCAAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTg                                  >  2:362761/1‑90 (MQ=255)                                                  cAATGTTTATACCCAGCCATTGAATACGGCATGCGGTCCAAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTg                                  >  2:624584/1‑90 (MQ=255)                                                   aaTGTTTATACCCAGCCATTGAATGCGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGAt                                >  2:142894/1‑90 (MQ=255)                                                   aaTGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGAt                                >  2:54182/1‑90 (MQ=255)                                                   aaTGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGAt                                >  2:890939/1‑90 (MQ=255)                                                   aaTGTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGAt                                >  1:966024/1‑90 (MQ=255)                                                      gTTTATACCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGtt                             <  2:259759/90‑1 (MQ=255)                                                       tttATACCCAGCCATTGAATACGGCATGCGGTCCAAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGtt                             <  1:624584/90‑1 (MQ=255)                                                       tttATACCCAGCCATTGAATACGGCATGCGGTCCAAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGtt                             <  1:362761/90‑1 (MQ=255)                                                         tataCCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTgg                                              <  1:769531/70‑1 (MQ=255)                                                         tataCCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTgg                                              >  2:769531/1‑70 (MQ=255)                                                           taCCCAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCAc                        <  1:54182/90‑1 (MQ=255)                                                              ccAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTAt                                                          >  1:727746/1‑53 (MQ=255)                                                              ccAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTAt                                                          <  2:727746/53‑1 (MQ=255)                                                              caaGCAATTGAATACGGCATGCGGTCC‑AAAAAAACAGGACGTAAAACGTGTATTCGATGATGCGGGGCCGCATAGTGATGTTTTCACTcc                     >  2:453919/3‑90 (MQ=255)                                                               cAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACt                       <  1:424912/87‑1 (MQ=255)                                                               cAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACt                       >  2:424912/1‑87 (MQ=255)                                                               cAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTccc                    >  2:439034/1‑90 (MQ=255)                                                               cAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGATGTgg                                              >  1:242074/1‑64 (MQ=255)                                                               cAGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGATGTgg                                              <  2:242074/64‑1 (MQ=255)                                                                aGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTcccc                   >  2:882382/1‑90 (MQ=255)                                                                aGCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGAATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTcccc                   >  1:476478/1‑90 (MQ=255)                                                                 gCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTCCCCt                  <  2:966024/90‑1 (MQ=255)                                                                 gCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTCCCCt                  <  2:823562/90‑1 (MQ=255)                                                                 gCCATTGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTCCCCt                  >  1:823562/1‑90 (MQ=255)                                                                      tGAATACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTCCCCTGATTc             <  2:103950/90‑1 (MQ=255)                                                                         aTACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTgg                                              >  2:1052093/1‑54 (MQ=255)                                                                         aTACGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTgg                                              <  1:1052093/54‑1 (MQ=255)                                                                           aCGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTCCCCTGAt               <  2:190396/83‑1 (MQ=255)                                                                           aCGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTCCCCTGAt               >  1:190396/1‑83 (MQ=255)                                                                           aCGGCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTCCCCTGATTCaattt        >  1:12501/1‑90 (MQ=255)                                                                              gCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGt                                           >  2:157389/1‑52 (MQ=255)                                                                              gCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGt                                           <  1:157389/52‑1 (MQ=255)                                                                              gCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAAt                                      >  2:455701/1‑57 (MQ=255)                                                                              gCATGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAAt                                      <  1:455701/57‑1 (MQ=255)                                                                                 tGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGtt                                                     >  2:1026249/1‑39 (MQ=39)                                                                                 tGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGtt                                                     <  1:1026249/39‑1 (MQ=255)                                                                                 tGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTCCCCTGATTCAATTTAAtttt  >  2:583772/1‑90 (MQ=255)                                                                                 tGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTCCCCTGATTCAATTTAAtttt  >  1:175446/1‑90 (MQ=255)                                                                                 tGCGGTCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTCCCCTGATTCAATTTAAtttt  >  2:946466/1‑90 (MQ=255)                                                                                     |                                                                                       ATAAATAATTAAAATATAAACATTGCAATACATTGATTCAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCC‑AAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTT  >  NZ_CP009273/2449142‑2449311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑