Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,495,699	A→G	S756P (TCC→CCC)	ptsP ←	phosphoenolpyruvate‑‑protein phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,495,699	0	A	G	100.0%	42.7 / NA	13	S756P (TCC→CCC)	ptsP	phosphoenolpyruvate‑‑protein phosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/8);  total (5/8)

CCAGCGTCAGCATGATCACCAGTTCGACTTCGCCCATTTCTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGAC  >  NZ_CP009273/2495612‑2495783                                                                                        |                                                                                     ccAGCGTCAGCATGATCACCAGTTCGACTTCGCCCATTTCTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGGAt                                                                                    >  1:754074/1‑90 (MQ=255)       tCAGCATGATCACCAGTTCGACTTCGCCCATTTCTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGGATGACGGa                                                                              >  1:607544/1‑90 (MQ=255)       tCAGCATGATCACCAGTTCGACTTCGCCCATTTCTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGGATGACGGa                                                                              <  2:568077/90‑1 (MQ=255)        cAGCATGATCACCAGTTCGACTTCGCCCATTTCTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGGATGACGGAt                                                                             <  2:754074/90‑1 (MQ=255)              gATCACCAGTTCGACTTCGCCCATTTCTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGGATGACGGATCCACTg                                                                       <  1:63582/90‑1 (MQ=255)                      gTTCGACTTCGCCCATTTCTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGGATGACGGATCCACTGAGATTTgg                                                               <  2:252147/90‑1 (MQ=255)                          gACTTCGCCCATTTCTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGGATGACGGATCCACTg                                                                       <  1:1048079/78‑1 (MQ=255)                          gACTTCGCCCATTTCTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGGATGACGGATCCACTg                                                                       >  2:1048079/1‑78 (MQ=255)                                        cTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGGATGACGGATCCACTGAGATTTGGTGTTCGGGATCGCTACGc                                             >  1:302994/1‑90 (MQ=255)                                             tGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGGATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCaaaa                                        >  2:684281/1‑90 (MQ=255)                                                    caATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGGATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGc                                 <  1:1050744/90‑1 (MQ=255)                                                                                  tACTGGGATGACGGAACCACTGAGATTTGGTGTGCGCGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGa   <  2:925433/90‑1 (MQ=255)                                                                                   ccTGGGATGACGGATCCACCGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGAc  <  2:607544/89‑1 (MQ=255)                                                                                        |                                                                                     CCAGCGTCAGCATGATCACCAGTTCGACTTCGCCCATTTCTGACTGCCAGCCAATCGGTTTCGCCAGCCGGGCAATGCTGATACTGGAATGACGGATCCACTGAGATTTGGTGTGCGGGATCGCTACGCCAAAACCAACGCCGGTGGTAACAATCTCTTCCCGCTGCCAGAC  >  NZ_CP009273/2495612‑2495783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑