| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,545,923 | A→G | G71G (GGA→GGG) | amiA → | N‑acetylmuramoyl‑L‑alanine amidase AmiA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,545,923 | 0 | A | G | 95.7% | 75.9 / ‑6.2 | 23 | G71G (GGA→GGG) | amiA | N‑acetylmuramoyl‑L‑alanine amidase AmiA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (11/11); minor base T (1/0); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGACACAGCAAGCCGAAAGCCAAAAAATCTGGCGGCAAACGTGTCGTTGTTCTCGATCCAGGTCACGGCGGAATTGATACCGGAGCGATCGGACGCAACGGTTCGAAAGAAAAACATGTGGTGCTGGCGATTGCTAAAAACGTCCGTTCCATTTTGCGTAATCATGGGAT > NZ_CP009273/2545839‑2546009 | cGGACACAGCAAGCCGAAAGCCAAAAAATCTGGCGGCAAACGTGTCGTTGTTCTCGATCCAGGTCACGGCGGAATTGATACCGGGGCGAt > 1:266241/1‑90 (MQ=255) acacAGCAAGCCGAAAGCCAAAAAATCTGGCGGCAAACGTGTCGTTGTTCTCGATCCAAGTCACGGCGGAATTGATACCGGGGCGATCgg > 2:525272/1‑90 (MQ=255) caGCAAGCCGAAAGCCAAAAAATCTGGCGGCAAACGTGTCGTTGTTCTCGATCCAGGTCACGGCGGAATTGATACCGGGGCGATCGGACg > 1:78747/1‑90 (MQ=255) aaGCCGAAAGCCAAAAAATCTGGCGGCAAACGTGTCGTTGTTCTCGATCCAGGTCACGGCGGAATTGATACCGGGGCGATCGGACGCAAc < 1:525272/90‑1 (MQ=255) aaGCCGAAAGCCAAAAAATCTGGCGGCAAACGTGTCGTTGTTCTCGATCCAGGTCACGGCGGAATTGATACCGGGGCGATCGGACGCAAc < 1:644376/90‑1 (MQ=255) tGGCGGCAAACGTGTCGTTGTTCTCGATCCAGGTCACGGCGGAATTGATACCGGGGCGATCGGACGCAACGGTTCGAAAGAAAAACAtgt < 2:764166/90‑1 (MQ=255) tGGAGGCAAACGTGTCGTTGTTCTCGATCCAGGTCACGGCGGAATTGATACCGGGGCGATCGGACGCAACGGTTCGAAAGAAAAACAtgt < 2:703817/90‑1 (MQ=255) gtgtCGTTGTTCTCGATCCAGGTCACGGCGGAATTGATACCGGGGCGATCGGACGCAACGGTTCGAAAGAAAAACATGTGGTGCTGGCGa > 1:646919/1‑90 (MQ=255) gtgtCGTTGTTCTCGATCCAGGTCACGGCGGAATTGATACCGGGGCGATCGGACGCAACGGTTCGAAAGAAAAACATGTGGTGCTGGCGa > 1:608475/1‑90 (MQ=255) tgtCGTTGTTCTCGATCCAGGTCACGGGGGAATTGATACCGGGGCGATCGGACGCAACGGTTCGAAAGAAAAACAtgt > 1:536972/1‑78 (MQ=255) tgtCGTTGTTCTCGATCCAGGTCACGGGGGAATTGATACCGGGGCGATCGGACGCAACGGTTCGAAAGAAAAACAtgt < 2:536972/78‑1 (MQ=255) tctcGATCCAGGTCACGGCGGAATTGATACCGGGGCGATCGGACGCAACGGTTCGAAAGAAAAACATGTGGTGCTGGCGATTGCTaaaaa > 2:593629/1‑90 (MQ=255) cAGGTCACGGCGGAATTGATACCGGGGCGATCGGACGCAACGGTTCGAAAGAAAAACATGTGGTGCTGGCGATTGCTAAAAACGTCCGtt > 1:210119/1‑90 (MQ=255) cAGGTCACGGCGGAATTGATACCGGGGCGATCGGACGCAACGGTTCGAAAGAAAAACATGTGGGGCTGGCGATTGCTAAAAACGTCCGtt > 2:31559/1‑90 (MQ=255) aCGGCGGAATTGATACCGGGGCGATCGGACGCAACGGTTCGAAAGAAAAACATGTGGTGCTGGCGATTGCTAAAAACGTCCGTTCCAttt < 1:314185/90‑1 (MQ=255) cggcggAATTGATACCGGGGCGATCGGACGCAACGGTTCGAAAGAAAAACATGTGGTGCTGGCGATTGCTGAAAACGTCCGTTCCAtttt > 1:1052309/1‑90 (MQ=255) cggcggAATTGATACCGGGGCGATCGGACGCAACGGTTCGAAAGAAAAACATGTGGTGCTGGCGATTGCTAAAAACGTCCGTTCCAtttt > 1:868827/1‑90 (MQ=255) ggAATTGATACCGGGGCGATCGGACGCAACGGTTCGAAAGAAAAACATGTGGTGCTGGCGATTGCTAAAAACGTCCGTTCCATTTTGCGt < 1:203553/90‑1 (MQ=255) ttGATACCGGGGCGATCGGACGCAACGGTTCGAAAGAAAAACATGTGGTGCTGGCGATTGCTAAAAACGTCCGTTCCATTTTGCGTAATc < 2:817086/90‑1 (MQ=255) ttGATACCGGGGCGATCGGACGCAACGGTTCGAAAGAAAAACATGTGGTGCTGGCGATTGCTAAAAACGTCCGTTCCATTTTGCGTAATc < 2:966240/90‑1 (MQ=255) gATACCGGGGCGATCGGACGCAACGGTTCGAAAGAAAAACATGTGGTGCTGGCGATTGCTAAAAACGTCCGTTCCATTTTGCGTAATCAt < 2:608475/90‑1 (MQ=255) gATACCGGGGCGATCGGACGCAACGGTTCGAAAGAAAAACATGTGGTGCTGGCGATTGCTAAAAACGTCCGTTCCATTTTGCGTAATCAt < 2:206917/90‑1 (MQ=255) cGGTGCGATCGGACGCGACGGTTCGAAAGAAAAACATGTGGAGCTGGCGATTGCTAAAAAAGCCCGTTCCTATATGCGTATTCATgggct > 2:673855/1‑88 (MQ=255) | CGGACACAGCAAGCCGAAAGCCAAAAAATCTGGCGGCAAACGTGTCGTTGTTCTCGATCCAGGTCACGGCGGAATTGATACCGGAGCGATCGGACGCAACGGTTCGAAAGAAAAACATGTGGTGCTGGCGATTGCTAAAAACGTCCGTTCCATTTTGCGTAATCATGGGAT > NZ_CP009273/2545839‑2546009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |