Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,549,285	T→G	T159P (ACC→CCC)	eutL ←	ethanolamine utilization microcompartment protein EutL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,549,285	0	T	G	100.0%	37.9 / NA	12	T159P (ACC→CCC)	eutL	ethanolamine utilization microcompartment protein EutL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (6/6);  total (6/6)

TGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGTGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTAC  >  NZ_CP009273/2549245‑2549368                                         |                                                                                    tGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGGCGCTTCCAGCGGCGGTGCCACCAGATACGCCATCGGATCGCCAAGCGTg                                    >  1:576580/1‑90 (MQ=255)  gTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGGCGCTTCCAGCGGCGGTGCCACCAGATACGCCATCGGATCGCCAAGCGTGa                                   <  2:731343/90‑1 (MQ=255)      gTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGGCGCTTCCAGCGGCGGTGCCCCCAGATACGCCATCGGATCGCCAAGCGGGATccc                               >  1:600235/1‑90 (MQ=255)        cGGCAGACTTCAGGGCTGCATCAATGCCATAGGGCGCTTCCAGCGGCGGTGCCCCCAGATACGCCCCCGGATCGCCAAACGGGAGccccg                             >  2:939790/1‑88 (MQ=255)                   aGGGCTGCATCAATGCCATAGGGCGCTTCCAGCGGCGGTGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATgag                  <  1:939790/90‑1 (MQ=255)                   aGGGCTGCATCAATGCCATAGGGCGCTTCCAGCGGCGGTGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATgag                  <  2:467315/90‑1 (MQ=255)                   aGGGCTGCATCAATGCCATAGGGCGCTTCCAGCGGCGGTGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATgag                  <  2:495994/90‑1 (MQ=255)                   aGGGCTGCATCAATGCCATAGGGCGCTTCCAGCGGCGGTGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATgag                  <  2:547581/90‑1 (MQ=255)                         gCATCAATGCCATAGGGCGCTTCCAGCGGCGGTGccac                                                                <  1:768782/38‑1 (MQ=38)                         gCATCAATGCCATAGGGCGCTTCCAGCGGCGGTGccac                                                                >  2:768782/1‑38 (MQ=38)                         gCATCAATGCCATAGGGCGCTTCCAGCGGCGGTGCCACCAGATACGCCATCGGATCCCCAAGCGGGATCCCCGCGGGTGGTGAGAGATaa            >  2:517353/1‑90 (MQ=255)                                   cATAGGGCGCTTCCAGCGGCGGTGCCACCAGATACGCCATCGGATCGCCAAGCGGGATCCCGGCGGTTTATGAGAGGTAAGCAACGGGAc  >  1:912310/1‑90 (MQ=255)                                         |                                                                                    TGTACGTCGGCAGACTTCAGGGCTGCATCAATGCCATAGGTCGCTTCCAGCGGCGGTGCCACCAGATACGCCATCGGATCGCCAAGCGTGATCCCGGCGGTTGATGAGAGATAAGAACCGGTAC  >  NZ_CP009273/2549245‑2549368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑