| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,550,898 | T→C | I381V (ATC→GTC) | eutB ← | ethanolamine ammonia‑lyase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,550,898 | 0 | T | C | 97.1% | 116.5 / ‑4.7 | 35 | I381V (ATC→GTC) | eutB | ethanolamine ammonia‑lyase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (17/17); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
ATGCGGTGGTCTGGTAGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGATCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGTGGTTGGTATAACAGCAGTCACAGCCCATAGAGATGCCGCTCAGCTT > NZ_CP009273/2550814‑2550982 | aTGCGGTGGTCTGGTAGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGAccatca > 2:786046/1‑90 (MQ=255) tGCGGTGGTCTGGTAGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAg < 2:202442/90‑1 (MQ=255) tGCGGTGGTCTGGTAGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAg < 1:584180/90‑1 (MQ=255) ggtCTGGTAGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGATGGCGAGCAGGACCATCAGGTTTTc < 1:459062/90‑1 (MQ=255) gtCTGGTAGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCg > 1:788651/1‑90 (MQ=255) ggTAGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGa < 2:802052/90‑1 (MQ=255) gTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGATCATCAGGTTTTCGTTGAGGtt < 2:532833/90‑1 (MQ=255) ttGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTc > 1:290459/1‑90 (MQ=255) tGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCt < 2:318194/90‑1 (MQ=255) cATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGgtc < 2:583423/90‑1 (MQ=255) gTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGgtc > 1:251358/1‑84 (MQ=255) gTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGgtc < 2:251358/84‑1 (MQ=255) tcaCCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGtcagcgtcagcg < 2:788651/90‑1 (MQ=255) ccAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGtggt > 2:581235/1‑90 (MQ=255) cAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGtggtt > 2:849479/1‑90 (MQ=255) cAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGtggtt > 1:916189/1‑90 (MQ=255) cAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGtggtt < 2:916189/90‑1 (MQ=255) aGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGa < 2:937458/64‑1 (MQ=255) aGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGa > 1:937458/1‑64 (MQ=255) aGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGgtc > 1:671774/1‑74 (MQ=255) aGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGgtc < 2:671774/74‑1 (MQ=255) aGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGtggttg < 2:290459/90‑1 (MQ=255) aGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTAAGCGtggttg < 2:432486/90‑1 (MQ=255) gCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGtggttgg > 1:892502/1‑90 (MQ=255) gCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGtggtt > 1:1048919/1‑88 (MQ=255) gCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGtggtt < 2:1048919/88‑1 (MQ=255) ccATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGTGGTTGGTATAAcagc < 1:1059846/90‑1 (MQ=255) tgatgTAGTTGCAGCCTGCGGTGGCTAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGTGGTTGGTATAACAGCAGt > 1:448733/1‑90 (MQ=255) tgTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGTGGTTGGTATAACAGCAGTcac < 2:763436/90‑1 (MQ=255) tgTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGTGGTTGGTATAACAGCAGTcac > 2:256733/1‑90 (MQ=255) tgTAGTTGCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGTGGTTGGTATAACAGCAGTcac > 2:799911/1‑90 (MQ=255) tAGTTGCATCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGTGGTTGGTATAACAGCAGTCACAg > 1:251503/1‑90 (MQ=255) gCAGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGTGGTTGGTATAACAGCAGTCACAGCCCAt > 1:895231/1‑90 (MQ=255) aGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGTGGTTGGTATAACAGCAGTCACAGCCCATag > 1:954030/1‑90 (MQ=255) aGCCTGCGGTGGCGAGCAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGTGGTTGGTATAACAGCAGTCACAGCCCATag > 1:819490/1‑90 (MQ=255) cAGGACCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGTGGTTGGTATAACAGCAGTCACAGCCCATAGAGATGCCGCTCAGCtt < 2:251503/90‑1 (MQ=255) | ATGCGGTGGTCTGGTAGTTGAGCATGATGTCATCACCCAGCGGCATCCCCATGATGTAGTTGCAGCCTGCGGTGGCGAGCAGGATCATCAGGTTTTCGTTGAGGTTCTGGTCAGCGTCAGCGTGGTTGGTATAACAGCAGTCACAGCCCATAGAGATGCCGCTCAGCTT > NZ_CP009273/2550814‑2550982 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |