Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,929,425	C→T	R161R (CGC→CGT)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,929,425	0	C	T	100.0%	84.0 / NA	32	R161R (CGC→CGT)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/9);  total (23/9)

CTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGT  >  NZ_CP009273/2929350‑2929509                                                                            |                                                                                     ctcCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCgg                                                                        >  2:193911/1‑90 (MQ=255) ctcCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCgg                                                                        >  1:501961/1‑90 (MQ=255) ctcCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCgg                                                                        >  1:507535/1‑90 (MQ=255)  tcCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGt                                                                       >  2:210095/1‑90 (MQ=255)        tACGGTCATGACGTTCAGGATTCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGcc                                                                 <  1:393083/90‑1 (MQ=255)        tACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGcc                                                                 >  2:846687/1‑90 (MQ=255)         aCGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCg                                                                >  1:315926/1‑90 (MQ=255)           ggTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCGTc                                                              >  2:901669/1‑90 (MQ=255)           ggTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTACGTTTTGCACGCGCCGGTTTGGCCggc                                                              >  2:620118/1‑88 (MQ=255)             tCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCGTCGc                                                            >  1:318731/1‑90 (MQ=255)             tCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTCCCCGCGCCGGTTTGGCCGTCGc                                                            >  1:5330/1‑90 (MQ=255)                     ttCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGa                                                    >  2:817711/1‑90 (MQ=255)                      tCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGcc                                                                 <  2:983024/76‑1 (MQ=255)                      tCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGcc                                                                 >  1:983024/1‑76 (MQ=255)                      tCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGaa                                                   <  2:898538/90‑1 (MQ=255)                      tCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGaa                                                   >  1:448397/1‑90 (MQ=255)                      tCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGaa                                                   <  1:210095/90‑1 (MQ=255)                      tCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGaa                                                   <  1:193911/90‑1 (MQ=255)                      tCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGaa                                                   >  2:738844/1‑90 (MQ=255)                            tGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTaa                                             >  2:179368/1‑90 (MQ=255)                            tGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTaa                                             >  2:723098/1‑90 (MQ=255)                              ccGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTaaaa                                           >  2:383272/1‑90 (MQ=255)                                   gACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTAt                                      >  1:401683/1‑90 (MQ=255)                                   gACACATCGAGTCCTGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGGTTGGCCGTGGCCAGCATGAAAGGTAAAAGCTAt                                      >  2:785235/1‑90 (MQ=255)                                                 tGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCGTCGCCa                                                          <  1:310448/56‑1 (MQ=255)                                                 tGCCGATGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCGTCGCCa                                                          >  2:310448/1‑56 (MQ=255)                                                      aTGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGaa                                                   >  2:163615/1‑58 (MQ=255)                                                      aTGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGaa                                                   <  1:163615/58‑1 (MQ=255)                                                      aTGTTGAAGAAAAACTGCTGCGTTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGttt                   >  1:2642/1‑90 (MQ=255)                                                                    ctgctgCGTTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGcc     <  1:738844/90‑1 (MQ=255)                                                                     tgctgcGTTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCg    >  2:469288/1‑90 (MQ=255)                                                                       ctgcGTTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGt  <  1:104445/90‑1 (MQ=255)                                                                            |                                                                                     CTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGT  >  NZ_CP009273/2929350‑2929509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑