Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	232,843	T→C	I97T (ATC→ACC)	dnaQ →	DNA polymerase III subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	232,843	0	T	C	100.0%	108.8 / NA	31	I97T (ATC→ACC)	dnaQ	DNA polymerase III subunit epsilon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/19);  total (12/19)

GCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAAT  >  NZ_CP009273/232758‑232919                                                                                      |                                                                             gCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCAt                                                                          >  1:1071194/1‑90 (MQ=255)      tGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGc                                                                     >  2:965813/1‑90 (MQ=255)      tGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGc                                                                     >  1:162278/1‑90 (MQ=255)      tGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGc                                                                     >  2:89268/1‑90 (MQ=255)      tGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGc                                                                     <  1:518938/90‑1 (MQ=255)      tGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGc                                                                     <  1:595661/90‑1 (MQ=255)      tGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGc                                                                     >  2:496437/1‑90 (MQ=255)      tGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGc                                                                     >  2:233089/1‑90 (MQ=255)        aaTTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAg                                                                   <  2:224481/90‑1 (MQ=255)                  cGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGatat                                                         <  2:534491/90‑1 (MQ=255)                   gATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATc                                                        >  2:394321/1‑90 (MQ=255)                   gATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATc                                                        <  2:120143/90‑1 (MQ=255)                       aGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCt                                                    >  2:440269/1‑90 (MQ=255)                           cACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTAt                                                <  2:829429/90‑1 (MQ=255)                                     gAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAg                                      <  2:995450/90‑1 (MQ=255)                                     gAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAg                                      <  1:448851/90‑1 (MQ=255)                                       aGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAg                                                                   <  1:774431/59‑1 (MQ=255)                                       aGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAg                                                                   >  2:774431/1‑59 (MQ=255)                                                       aTGGCCTATATTCGCGGCGCGGAGTTGGTGACCCATTACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcgc                    <  2:171059/90‑1 (MQ=255)                                                       aTGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcgc                    <  2:1071194/90‑1 (MQ=255)                                                       aTGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcgc                    <  1:1020920/90‑1 (MQ=255)                                                             tataTTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAtt              >  1:864121/1‑90 (MQ=255)                                                             tataTTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATAtt              >  2:991663/1‑90 (MQ=255)                                                                 ttCTCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa          <  2:215669/90‑1 (MQ=255)                                                                 ttCGCGGCGCGGAGTTGGTGACCCATAACGCATCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa          <  2:579809/90‑1 (MQ=255)                                                                 ttCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa          <  2:162278/90‑1 (MQ=255)                                                                 ttCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa          <  2:244042/90‑1 (MQ=255)                                                                 ttCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa          <  2:697218/90‑1 (MQ=255)                                                                 ttCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa          <  1:250487/90‑1 (MQ=255)                                                                     cggcgcggAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGAc      <  1:115855/90‑1 (MQ=255)                                                                         gcggAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAAt  >  1:75001/1‑90 (MQ=255)                                                                                      |                                                                             GCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAAT  >  NZ_CP009273/232758‑232919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑