| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,972,615 | A→G | D79G (GAT→GGT) | lysR → | DNA‑binding transcriptional regulator LysR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,972,615 | 0 | A | G | 78.1% | 57.3 / 13.3 | 32 | D79G (GAT→GGT) | lysR | DNA‑binding transcriptional regulator LysR |
| Reads supporting (aligned to +/- strand): ref base A (6/1); new base G (12/13); total (18/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-01 | |||||||||||
CGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCTTT > NZ_CP009273/2972548‑2972702 | cGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGaa < 2:119713/90‑1 (MQ=255) aTTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAg > 1:1074899/1‑90 (MQ=255) tCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTgcg > 1:733598/1‑90 (MQ=255) cTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGcgcg < 1:497739/90‑1 (MQ=255) aCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGGTCGCATTGTCAGAGCCGCAGAAAGTCTGCGCGAg > 2:14254/1‑90 (MQ=255) aCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCGTTGTCAGGTACGCAGAAAGTCTGCGCGAg > 2:521236/1‑90 (MQ=255) aCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAg > 1:87128/1‑90 (MQ=255) gTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGttt > 1:430120/1‑90 (MQ=255) gTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGttt > 1:537213/1‑90 (MQ=255) tGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTc > 2:472647/1‑90 (MQ=255) cAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGGTCGCATTGTCAGCGGCGCAGAAAGTCTGCGCGAGTTTCGc < 1:74709/90‑1 (MQ=255) cAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGc < 2:507861/90‑1 (MQ=255) aCTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAggg < 1:319171/90‑1 (MQ=255) tCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACt < 2:735632/90‑1 (MQ=255) agTGCAACGATCCTGGTACGGACTGGGTCGCATTGTCAGAGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATtgcctg < 1:14254/90‑1 (MQ=255) aaCGATCCTGGTACGGACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAAc < 2:791999/72‑1 (MQ=255) aaCGATCCTGGTACGGACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAAc > 1:791999/1‑72 (MQ=255) aTCCTGGTACGGACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACt < 1:645437/69‑1 (MQ=255) aTCCTGGTACGGACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACt > 2:645437/1‑69 (MQ=255) aTCCTGGTACGGACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTCCCTGCCTTCCGGt > 2:112749/1‑90 (MQ=255) ggACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGc < 1:658239/88‑1 (MQ=255) ggACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGc > 2:658239/1‑88 (MQ=255) ggACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCaa < 1:682663/90‑1 (MQ=255) ggACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCaa < 2:173609/90‑1 (MQ=255) ggACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCaa < 1:481381/90‑1 (MQ=255) ggACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCaa < 1:472647/90‑1 (MQ=255) ggACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCaa > 1:173609/1‑90 (MQ=255) gACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAAt > 1:78010/1‑90 (MQ=255) gACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAAt > 1:588174/1‑90 (MQ=255) gACTGGGTCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAAt > 1:131812/1‑90 (MQ=255) gACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAAt > 2:355171/1‑90 (MQ=255) ggATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCttt > 1:39650/1‑90 (MQ=255) | CGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCTTT > NZ_CP009273/2972548‑2972702 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |