Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,427,745	A→G	E58G (GAA→GGA)	fmt →	methionyl‑tRNA formyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,427,745	0	A	G	100.0%	114.0 / NA	32	E58G (GAA→GGA)	fmt	methionyl‑tRNA formyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/16);  total (16/16)

GTTCACCCAGCCAGACCGACCGGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGAAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGAAAACCAGCAACTGGTCGCCGAACTGCAGGCTGATGTTATG  >  NZ_CP009273/3427671‑3427830                                                                           |                                                                                      gtTCACCCAGCCAGACCGACCGGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGt                                                                        >  2:416736/1‑90 (MQ=255) gtTCACCCAGCCAGACCGACCGGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGt                                                                        >  2:418285/1‑90 (MQ=255) gtTCACCCAGCCAGACCGACCGGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGt                                                                        >  1:319359/1‑90 (MQ=255) gtTCACCCAGCCAGACCGACCGGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGGCTGCCCGt                                                                        >  2:136693/1‑90 (MQ=255)   tCACCCAGCCAGACCGACCGGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGgaaaa                                                                                   <  1:362954/77‑1 (MQ=255)   tCACCCAGCCAGACCGACCGGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGgaaaa                                                                                   >  2:362954/1‑77 (MQ=255)      cccAGCCAGACCGACCGGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGATGAGGGAAAAGGTCTGCCCGTTTTTc                                                                   <  2:707626/90‑1 (MQ=255)        cagccagACCGACCGGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCaa                                                                 <  1:115693/90‑1 (MQ=255)           ccagACCGACCGGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCt                                                              >  1:709850/1‑90 (MQ=255)              gaccgaccGGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTc                                                                   <  2:69376/82‑1 (MQ=255)              gaccgaccGGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTc                                                                   >  1:69376/1‑82 (MQ=255)              gaccgaccGGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGtt                                                           <  1:416736/90‑1 (MQ=255)              gaccgaccGGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGtt                                                           <  1:418285/90‑1 (MQ=255)              gaccgaccGGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGtt                                                           <  2:976792/90‑1 (MQ=255)                 cgaccgGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTcc                                                        <  1:84355/90‑1 (MQ=255)                  gaccgGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTccc                                                       >  2:751748/1‑90 (MQ=255)                                ggTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGaa                                         <  1:837531/90‑1 (MQ=255)                                ggTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGaa                                         <  2:319359/90‑1 (MQ=255)                                ggTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGAGTCCACAAGaa                                         <  1:412113/90‑1 (MQ=255)                                 gTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGaaa                                        >  1:931526/1‑90 (MQ=255)                                   aaaaaaCTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGAAAAc                                      <  1:297846/90‑1 (MQ=255)                                           gATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGAAAACCAGCAACt                              >  1:953929/1‑90 (MQ=255)                                           gATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGAAAACCAGCAACt                              >  1:793465/1‑90 (MQ=255)                                             tGCCCAGCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGAAAACCAGCAACTgg                            >  1:226361/1‑90 (MQ=255)                                                   gCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGAAAACCAGCAACTGGTCGCCg                      >  1:650863/1‑90 (MQ=255)                                                   gCCCGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGAAAACCAGCAACTGGTCGCCg                      <  1:253095/90‑1 (MQ=255)                                                    cccGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGAAAACCAGCAACTGGTCGCCGa                     <  2:650863/90‑1 (MQ=255)                                                      cGGTTAAAGTTCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGAAAACCAGCAACTGGTCGCCGAAc                   <  2:74593/90‑1 (MQ=255)                                                                tCTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGAAAACCAGCAACTGGTCGCCGAACTGCAGGCTGa         <  2:516023/90‑1 (MQ=255)                                                                 cTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGAAAACCAGCAACTGGTCGCCGAACTGCAGGCTGAt        >  2:87469/1‑90 (MQ=255)                                                                 cTGGCTGAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGAAAACCAGCAACTGGTCGCCGAACTGCAGGCTGAt        >  1:154470/1‑90 (MQ=255)                                                                       gAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGAAAACCAGCAACTGGTCGCCGa                     <  1:462952/71‑1 (MQ=255)                                                                       gAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGAAAACCAGCAACTGGTCGCCGa                     >  2:462952/1‑71 (MQ=255)                                                                       gAGGGAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGAAAACCAGCAACTGGTCGCCGAACTGCAGGCTGATGTTATg  >  2:629213/1‑90 (MQ=255)                                                                           |                                                                                      GTTCACCCAGCCAGACCGACCGGCAGGACGCGGTAAAAAACTGATGCCCAGCCCGGTTAAAGTTCTGGCTGAGGAAAAAGGTCTGCCCGTTTTTCAACCTGTTTCCCTGCGTCCACAAGAAAACCAGCAACTGGTCGCCGAACTGCAGGCTGATGTTATG  >  NZ_CP009273/3427671‑3427830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑