Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,620,259	A→T	D880E (GAT→GAA)	rbbA ←	ribosome‑associated ATPase/putative transporter RbbA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,620,259	0	A	T	100.0%	121.9 / NA	46	D880E (GAT→GAA)	rbbA	ribosome‑associated ATPase/putative transporter RbbA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (26/20);  total (26/20)

TTTTTTCAGCAGCAGGATACTTAAGCCCATCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGATCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCAACGTCCAGGCC  >  NZ_CP009273/3620175‑3620341                                                                                     |                                                                                   ttttttCAGCAGCAGGATACTTAAGCCCATCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAg                                                                               <  1:869582/90‑1 (MQ=255)  tttttCAGCAGCAGGATACTTAAGCCCATCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAgc                                                                              <  1:183362/90‑1 (MQ=255)              agGATACTTAAGCCCATCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAAc                                                                  <  2:930863/90‑1 (MQ=255)                 aTACTTAAGCCCATCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTc                                                               >  1:945062/1‑90 (MQ=255)                  tACTTAAGCCCATCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTcc                                                              <  2:345314/90‑1 (MQ=255)                   aCTTAAGCCCATCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTccc                                                             >  2:582731/1‑90 (MQ=255)                      tAAGCCCATCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCcgcg                                                          >  2:987179/1‑90 (MQ=255)                      tAAGCCCATCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCcgcg                                                          >  2:341353/1‑90 (MQ=255)                      tAAGCCCATCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCcgcg                                                          <  1:858203/90‑1 (MQ=255)                       aaGCCCATCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCgg                                                         >  2:386686/1‑90 (MQ=255)                          cccATCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCg                                                      >  1:60455/1‑90 (MQ=255)                           ccATCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGa                                                     >  1:50794/1‑90 (MQ=255)                            cATCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGAt                                                    <  1:582731/90‑1 (MQ=255)                            cATCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGAt                                                    >  2:594416/1‑90 (MQ=255)                            cATCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGAGGGCGAt                                                    >  1:19781/1‑90 (MQ=255)                             aTCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGAt                                                    <  2:976871/89‑1 (MQ=255)                             aTCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGAt                                                    >  1:976871/1‑89 (MQ=255)                              tCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAg                                                  >  1:994680/1‑90 (MQ=255)                                  cAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAg                                              >  2:394044/1‑90 (MQ=255)                                  cAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGACAg                                              >  2:742488/1‑90 (MQ=255)                                  cAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGAGGGCGATAGTCAg                                              <  2:19781/90‑1 (MQ=255)                                   aGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGa                                             <  2:952740/90‑1 (MQ=255)                                         aTGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGa                                       <  1:723649/90‑1 (MQ=255)                                           ggCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTTAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACt                                     >  1:556909/1‑90 (MQ=255)                                           ggCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACt                                     <  2:716018/90‑1 (MQ=255)                                             cTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTgg                                   <  2:60455/90‑1 (MQ=255)                                              tATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGt                                  >  1:6552/1‑90 (MQ=255)                                                     aaCGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAgc                                                                              >  2:325574/1‑39 (MQ=255)                                                     aaCGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAgc                                                                              <  1:325574/39‑1 (MQ=39)                                                     aaCGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTaa                           >  2:683003/1‑90 (MQ=255)                                                      aCGGGATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCggggtaa                          >  2:885544/1‑86 (MQ=255)                                                         ggATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCt                       >  1:941281/1‑90 (MQ=255)                                                         ggATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCt                       >  2:977812/1‑90 (MQ=255)                                                          gATAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTc                      >  1:364925/1‑90 (MQ=255)                                                           aTAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCg                     >  1:1030698/1‑90 (MQ=255)                                                           aTAAAAAGTTGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCg                     >  1:158419/1‑90 (MQ=255)                                                                   ttGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCa             >  2:1013658/1‑90 (MQ=255)                                                                   ttGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCa             <  1:266332/90‑1 (MQ=255)                                                                    tGCCACAAATCAGTTAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCaa            <  2:556909/90‑1 (MQ=255)                                                                    tGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCaa            <  2:667306/90‑1 (MQ=255)                                                                    tGCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCaa            <  1:1011043/90‑1 (MQ=255)                                                                    ggtcacaAAGCAGGCAGTTCCGGCGCTTTCCGGGACGACCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAGCCTCGCCGAACCaa            <  2:121804/87‑1 (MQ=255)                                                                     gCCACAAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCAAc           <  1:394044/90‑1 (MQ=255)                                                                       cacaAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCAACGt         >  2:607577/1‑90 (MQ=255)                                                                         caAATCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCAACGTcc       <  2:790818/90‑1 (MQ=255)                                                                            aTCAGTCAGTTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCAACGTCCAgg    >  2:161545/1‑90 (MQ=255)                                                                              cagtcagtTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCAACGTCCAGGcc  <  2:253930/90‑1 (MQ=255)                                                                              cagtcagtTCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCAACGTCCAGGcc  <  2:994680/90‑1 (MQ=255)                                                                                     |                                                                                   TTTTTTCAGCAGCAGGATACTTAAGCCCATCACCAGCGGGATGGCTATCAGTAACGGGATAAAAAGTTGCCACAAATCAGTCAGATCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCAACGTCCAGGCC  >  NZ_CP009273/3620175‑3620341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑