Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,845,141	A→G	I335T (ATC→ACC)	ilvB ←	acetolactate synthase large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,845,141	0	A	G	100.0%	124.8 / NA	35	I335T (ATC→ACC)	ilvB	acetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/21);  total (14/21)

GCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATC  >  NZ_CP009273/3845054‑3845226                                                                                        |                                                                                      gCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTc                                                                                     >  1:1001489/1‑90 (MQ=255)  cTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCa                                                                                    <  1:432507/90‑1 (MQ=255)    ttCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAAc                                                                                  <  1:656611/90‑1 (MQ=255)              gACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCa                                                                        <  1:499780/90‑1 (MQ=255)              gACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGAGCCAGCa                                                                        <  1:3798/90‑1 (MQ=255)               acacGGAAACTCCCGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCAc                                                                       <  2:86640/90‑1 (MQ=255)               acacGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCAc                                                                       <  2:140231/90‑1 (MQ=255)                      accTCACGCTGCAAATCCGCTACCAACTGGTGCCCCTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTTCGCCAGCACGtcatca                                                                <  2:1060254/88‑1 (MQ=255)                      aaCTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGGCAACTGCGCCAGCACGtcatca                                                                >  2:316594/1‑90 (MQ=255)                               tGCAAACCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCAACAGCGGGGTCAACTGCGCCAGCACGTCCTCAACGTCCGcc                                                       <  2:250197/90‑1 (MQ=255)                                  aaaTCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGa                                                    >  2:9838/1‑90 (MQ=255)                                        gCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCTcc                                              >  1:313996/1‑90 (MQ=255)                                              aaCTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGc                                        >  1:201480/1‑90 (MQ=255)                                                cTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCgg                                      <  1:422127/90‑1 (MQ=255)                                                  ggTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGgct                                    >  1:975681/1‑90 (MQ=255)                                                  ggTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGgct                                    >  2:625883/1‑90 (MQ=255)                                                    tGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGgctgc                                  <  2:201480/90‑1 (MQ=255)                                                      ccACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCtt                                <  2:1034257/90‑1 (MQ=255)                                                       cACTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTg                               >  1:632722/1‑90 (MQ=255)                                                        aCTCTGCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGa                              >  1:691225/1‑90 (MQ=255)                                                          tctGCACGCGGTTGCGCTTCCACCATCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCtt                                >  2:437801/1‑86 (MQ=255)                                                          tctGCACGCGGTTGCGCTTCCACCATCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCtt                                <  1:437801/86‑1 (MQ=255)                                                             gCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTa                         >  1:947228/1‑90 (MQ=255)                                                             gCACGCGGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTa                         >  1:43466/1‑90 (MQ=255)                                                                cgcgGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGATGCTTGATTTTAccc                      <  1:463735/90‑1 (MQ=255)                                                                  cgGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCtt                                >  1:650013/1‑78 (MQ=255)                                                                  cgGTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCtt                                <  2:650013/78‑1 (MQ=255)                                                                   ggTTGCGCTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGc                   <  2:1001489/90‑1 (MQ=255)                                                                          cTTCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCAc            <  2:405383/90‑1 (MQ=255)                                                                            tCCACCAGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACgg          >  2:501980/1‑90 (MQ=255)                                                                              caccaGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTc        <  2:281090/90‑1 (MQ=255)                                                                              caccaGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTc        <  1:9838/90‑1 (MQ=255)                                                                              caccaGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTc        <  2:883101/90‑1 (MQ=255)                                                                              caccaGCGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTc        <  1:971290/90‑1 (MQ=255)                                                                                    cGGGGTCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATc  <  2:152332/90‑1 (MQ=255)                                                                                        |                                                                                      GCTTTCGGGATTGGACACGGAAACTCACGCTGCAAATCCGCTACCAACTGGTGCCACTCTGCACGCGGTTGCGCTTCCACCAGCGGGATCAACTGCGCCAGCACGTCATCAACATCCGCCTGAATCGCCACGTGCGGCTGCTTGATTTTACCCAGCTCTGCACGGTCGATATC  >  NZ_CP009273/3845054‑3845226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑