| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 245,728 | A→G | pseudogene (830/1713 nt) | BW25113_RS01160 ← | flagellar biosynthesis protein FlhA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 245,728 | 0 | A | G | 87.0% | 55.4 / 1.4 | 23 | pseudogene (830/1713 nt) | BW25113_RS01160 | flagellar biosynthesis protein FlhA |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base G (10/10); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
CTTCATCAGCCTTTATGCCGTTGATGAAAATGGCGTACTGACTGGGCTTAAGGCGGAAGTTTTCCCGAATGCGGATCTCCGGCAGCAGCACGCCGTTGCCGTCAGAAATCACCTGCCGCACGCCGCGAATCCGCTGGGTGAGCGGGT > NZ_CP009273/245654‑245800 | cTTCATCAGCCTTTATGCCGTTGATGAAAATGGCGTACTGACTGGGCTTAAGGCGGAAGTTTTCCCGAATGCGGGTCTCCGgcagcagca > 1:311769/1‑90 (MQ=255) ttATGCCGTTGATGAAAATGGCGTACTGACTGGGCTTAAGGCGGAAGTTTTCCCGAATGCGGGTCTCCGGCAGCAGCACGCCGTTGCCGt > 2:229586/1‑90 (MQ=255) tATGCCGTTGATGAAAATGGCGTACTGACTGGGCTTAAGGCGGAAGTTTTCCCGAATGCGGGTCTCCGGCAGCAGCACGCCGTTGCCGTc < 1:242675/90‑1 (MQ=255) cGTTGATGAAAATGGCGTACTGACTGGGCTTAAGGCGGAAGTTTTCCCGAATGCGGGTCTCCGGCAGCAGCACGCCGTTGCCGTCAGaaa > 2:555567/1‑90 (MQ=255) cGTTGATGAAAATGGCGTACTGACTGGGCTTAAGGCGGAAGTTTTCCCGAATGCGGGTCTCCGGCAGCAGCACGCCGTTGCCGTCAGaaa > 2:383112/1‑90 (MQ=255) cGTTGATGAAAATGGCGTACTGACTGGGCTTAAGGCGGAAGTTTTCCCGAATGCGGATCTCCGGCAGCAGCACGCCGTTGCCGTCAGaaa > 2:643283/1‑90 (MQ=255) gCGTACTGACTGGGCTTAAGGCGGAAGTTTTCCCGAATGCGGGTCTCCGGCAGCAGCACGCCGTTGCCGTCAGAAATCACCTGCCGCAcg > 2:411966/1‑90 (MQ=255) gTACTGACTGGGCTTAAGGCGGAAGTTTTCCCGAATGCGGATCTCCGGCAGCAGCACGCCGTTGCCGTCAGAAATCACCTGCCGCAcgcc < 1:643283/90‑1 (MQ=255) actgactgGGCTTAAGGCGGAAGTTTTCCCGAATGCGGGTCTCCGGCAGCAGCACGCCGTTGCCGTCAGAAATCACCTGCCGCAcgccgc < 1:572276/90‑1 (MQ=255) tgGGCTTAAGGCGGAAGTTTTCCCGAATGCGGGTCTCCGgc > 2:723844/1‑41 (MQ=255) tgGGCTTAAGGCGGAAGTTTTCCCGAATGCGGGTCTCCGgc < 1:723844/41‑1 (MQ=255) tgGGCTTAAGGCGGAAGTTTTCCCGAATGCGGGTCTCCGGCAGCAGCACGCCGTTGCCGTCAGAAATCACCTGCCGCACGCCGCGAATcc > 1:512045/1‑90 (MQ=255) ggCTTAAGGCGGAAGTTTTCCCGAATGCGGGTCTCCGGCAGCAGCACGCCGTTGCCGTCAGAAATCACCTGCCGCACGCCGCGAATCCGc > 2:454708/1‑90 (MQ=255) ttAAGGCGGAAGTTTTCCCGAATGCGGGTCTCCGGCAGCAGCACGCCGTTGCCGTCAGAAATCACCTGCCGCACGCCGCGa < 1:1049127/81‑1 (MQ=255) ttAAGGCGGAAGTTTTCCCGAATGCGGGTCTCCGGCAGCAGCACGCCGTTGCCGTCAGAAATCACCTGCCGCACACCGCGa > 2:1049127/1‑81 (MQ=255) aaGGCGGAAGTTTTCCCGAATGCGGATCTCCGGCAGCAGCACGCCGTTGCCGTCAGAAATCACCTGCCGCACGCCGCGAATCCGCTGGGt > 2:386456/1‑90 (MQ=255) ggCGGAAGTTTTCCCGAATGCGGGTCTCCGGCAGCAGCACGCCGTTGCCGTCAGAAATCACCTGCCGCACGCCGCGAATCCGCTGGGTGa < 1:383112/90‑1 (MQ=255) gCGGAAGTTTTCCCGAATGCGGGTCTCCGGCAGCAGCACGCCGTTGCCGTCAGAAATCACCTGCCGCACGCCGCGAATCCGCTGGGTGAg < 2:397222/90‑1 (MQ=255) gCGGAAGTTTTCCCGAATGCGGGTCTCCGGCAGCAGCACGCCGTTGCCGTCAGAAATCACCTGCCGCACGCCGCGAATCCGCTGGGTGAg < 1:443436/90‑1 (MQ=255) gCGGAAGTTTTCCCGAATGCGGGTCTCCGGCAGCAGCACGCCGTTGCCGTCAGAAATCACCTGCCGCACGCCGCGAATCCGCTGGGTGAg < 1:411966/90‑1 (MQ=255) gCGGAAGTTTTCCCGAATGCGGGTCTCCGGCAGCAGCACGCCGTTGCCGTCAGAAATCACCTGCCGCACGCCGCGAATCCGCTGGGTGAg < 1:1065623/90‑1 (MQ=255) cGGAAGTTTTCCCGAATGCGGGTCTCCGGCAGCAGCACGCCGTTGCCGTCAGAAATCACCTGCCGCACGCCGCGAATCCGCTGGGTGAGc > 2:465929/1‑90 (MQ=255) aGTTTTCCCGAATGCGGGTCTCCGGCAGCAGCACGCCGTTGCCGTCAGAAATCACCTGCCGCACGCCGCGAATCCGCTGGGTGAGCGGGt < 2:252773/90‑1 (MQ=255) | CTTCATCAGCCTTTATGCCGTTGATGAAAATGGCGTACTGACTGGGCTTAAGGCGGAAGTTTTCCCGAATGCGGATCTCCGGCAGCAGCACGCCGTTGCCGTCAGAAATCACCTGCCGCACGCCGCGAATCCGCTGGGTGAGCGGGT > NZ_CP009273/245654‑245800 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |